Molecular Characterization of DNA-A Component of Horse Gram Yellow Mosaic Virus (HgYMV) from Southern India

Background: French bean (Phaseolus vulgaris L.), is one of the important legume vegetables grown in India. The disease mung bean yellow mosaic virus (MYMV) limits successful production of beans. MYMV has become an epidemic in bean growing regions, particularly in the tropical and subtropical countries. In summer, the disease is more serious and widely distributed and reported in various countries. The current study is aimed at identification of MYMV and molecular characterization of mung bean yellow mosaic virus in French bean. This study will be helpful for early detection of the virus, so that better preventive measures can be taken to control MYMV.Methods: The investigations were conducted during 2016-2017, all the agro climatic zones of Karnataka province were surveyed. The coat protein (CP) gene of MYMV was amplified using gene specific primer with DNA isolated from MYMV infected leaves samples in french bean. Polymerase chain reaction products were Sequenced and analysed using a bioinformatic tool. Result: The CP gene decrypt sequences analysis revealed that the identity and similarity in global alignment for all the obtained sequences ranging from 80.8 to 95.3% with reference to MYMV, whereas for horse gram yellow mosaic virus (HYMV) it ranges from 90.4 to 99.1%. Two distinct yellow mosaic viruses infecting french bean (MYMV and HYMV) were identified and it was observed that there exists considerable genetic variation among these species. Present work showed that the CP region is efficient enough to provide a simple, rapid and reliable method for early detection of MYMV infections in french bean, which would help to develop proper management strategies to control these viruses.

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Identification and Molecular Characterization of the Chloroplast Targeting Domain of Turnip yellow mosaic virus Replication Proteins

Frontiers in Plant Science ◽

10.3389/fpls.2017.02138 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 6

Author(s):

Lucille Moriceau ◽

Lucile Jomat ◽

Stéphane Bressanelli ◽

Catherine Alcaide-Loridan ◽

Isabelle Jupin

Keyword(s):

Mosaic Virus ◽

Molecular Characterization ◽

Virus Replication ◽

Yellow Mosaic Virus ◽

Turnip Yellow Mosaic Virus ◽

Replication Proteins ◽

Chloroplast Targeting

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Biological variability of zucchini yellow mosaic virus in Serbia

Pesticidi i fitomedicina ◽

10.2298/pif0904271v ◽

2009 ◽

Vol 24 (4) ◽

pp. 271-280 ◽

Cited By ~ 3

Author(s):

Ana Vucurovic ◽

Aleksandra Bulajic ◽

Ivana Djekic ◽

Danijela Ristic ◽

Janos Berenji ◽

...

Keyword(s):

Mosaic Virus ◽

Molecular Characterization ◽

Molecular Detection ◽

Zucchini Yellow Mosaic Virus ◽

Genomic Region ◽

Yellow Mosaic Virus ◽

Biological Study ◽

The Past ◽

Phenotypic Variations

Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world.

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