scholarly journals House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype

Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1418
Author(s):  
Ruperto González-Pérez ◽  
Paloma Poza-Guedes ◽  
Fernando Pineda ◽  
Miriam Castillo ◽  
Inmaculada Sánchez-Machín
Keyword(s):  
Atopic Dermatitis ◽  
Molecular Diagnosis ◽  
House Dust ◽  
House Dust Mite ◽  
Molecular Profile ◽  
Serum Samples ◽  
Prick Test ◽  
Dust Mite ◽  
Allergic Condition ◽  
Precise Diagnosis

Atopic dermatitis (AD) endotyping might be important for developing personalized diagnostic and therapeutic strategies to the different phenotypes. The current study investigated the IgE molecular profile to Dermatophagoides pteronyssinus (D. pteronyssinus) in a subset of patients afflicted with varying severity stages of atopic dermatitis in a subtropical region subjected to a high perennial house dust mite (HDM) exposure. We selected patients showing a clinically relevant sensitization to HDM with mild-to-moderate and severe AD according to their basal Severity Scoring Atopic Dermatitis (SCORAD) index. Skin prick test (SPT) with standardized mite extracts, as well as a Precision Allergy Molecular Diagnosis (PAMD@) panel including nine different D. pteronyssinus allergens and the related protein allergenic characterization, were assessed in all serum samples. A total of 80 European American AD patients with the marked T2 endotype confirmed their eligibility for the study. Major allergens (Der p 23, Der p 2, and Der p 1) were present in more than 86% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 65%. A serodominant role for Der p 11 could not be quantitatively confirmed in the present cohort. The proposed component resolved diagnosis (CRD) panel appeared to be sufficient to obtain a precise D. pteronyssinus molecular diagnosis in AD patients subjected to a climate-dependent high-mite allergen exposure. The raised seroprevalence of IgE response to Der p 23 confirmed this constituent as a major D. pteronyssinus allergen in severe stages of atopic dermatitis. A clinically driven molecular approach appears to be essential to frame a more precise diagnosis and therapy of this heterogeneous allergic condition.

scholarly journals Profil Penderita Alergi Dengan Hasil Skin Prick Test TDR Positif di Poliklinik Alergi-Imunologi RSUP Prof. Dr. R. D. Kandou Manado Periode 2007-2009

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Novitasari .
Keyword(s):  
Atopic Dermatitis ◽  
Skin Prick Test ◽  
House Dust ◽  
House Dust Mite ◽  
Public Hospital ◽  
House Dust Mites ◽  
Dust Mites ◽  
Prick Test ◽  
Dust Mite ◽  
Immune Assay

Background: House dust mites are inhaled allergens and as a trigger of the onset of allergic diseases such as bronchial asthma, atopic dermatitis, conjunctivitis, and alergic rhinitis. Mites are the major allergenic components from house dust. House dust mite section containing allergens in the cuticle, the sex organs and the gastrointestinal tract. Approximately 80% of patients allergic to house dust mites have specific IgE antibodies against group I and II allergens that clinically associated with asthma, atopic dermatitis, alergic rhinitis. This IgE can be detected in serum by immune assay or on the skin with a skin allergy test.Purpose: To provide a profil picture of allergy with the result of skin prick test positive to housedust mites in allergy - immunology clinic medicine in public hospital Prof. Kandou period 2007 - 2009.Method and results : This study uses a retrospective descriptive method trough medical records at the allergy- immunology clinic in public hospital Prof. Kandou period 2007 - 2009. Result of this study, the number of allergy sufferers with positive results of skin prick test of house dust mite is 136 patients, with the highest prevalence in 2008. Earned women more than men, highest in the age group 51 - 60 years, most patients work as civil servants, and most diagnosis was found is bronchial asthma.Keyword : house dust mite, allergy, skin prick test.ABSTRAKLatar Belakang: Tungau Debu Rumah (TDR) merupakan alergen hirup dan sebagai faktor pencetus timbulnya penyakit alergi seperti asma bronkial, dermatitis atopik, konjungtivitis, dan rinitis alergik.1-4 Tungau merupakan komponen alergenik utama dari debu rumah. Bagian TDR yang mengandung alergen adalah kutikula, organ seks dan saluran cerna.1-3Kurang lebih 80% penderita alergi TDR mempunyai antibodi IgE spesifik terhadap alergen kelompok I dan II yang secara klinis berkaitan dengan penyakit asma, dermatitis atopik, dan rinitis alergika.2 IgE ini dapat dideteksi dalam serum melalui immune assay atau pada kulit dengan tes kulit alergi.6Tujuan: Untuk memberikan gambaran mengenai profil penderita alergi dengan hasil skin prick test TDR positif di Poliklinik Alergi-Imunologi RSUP Prof. Dr. R. D. Kandou periode 2007-2009.Metode dan Hasil: Penelitian ini menggunakan metode retrospektif deskriptif melalui rekam medik di Poliklinik Alergi-Imunologi RSUP Prof. Dr. R. D. Kandou Manado Periode 2007-2009. Dari hasil penelitian ini didapatkan Jumlah penderita alergi dengan hasil skin prick test TDR positif (+) sebanyak 136 penderita, dengan prevalensi terbanyak pada tahun 2008. Didapatkan perempuan lebih banyak dibandingkan laki-laki, terbanyak pada kelompok usia 51-60 tahun, sebagian besar penderita berprofesi sebagai (PNS), dan diagnosis terbanyak yang ditemukan adalah asma bronkial.Kata Kunci: TDR, Alergi, Skin Prick Test

scholarly journals Distribution of atopic conditions among attendants to specialized allergy center/ Kirkuk/ Iraqi

2020 ◽  
Vol 3 (2) ◽  
pp. 139-153
Author(s):  
Chinar Kanbar ◽  
Abdulameer Samad ◽  
Ali Galleb
Keyword(s):  
Allergic Rhinitis ◽  
Atopic Dermatitis ◽  
Environmental Factors ◽  
Skin Prick Test ◽  
House Dust ◽  
House Dust Mite ◽  
Atopic Disease ◽  
Atopic Diseases ◽  
Prick Test ◽  
Dust Mite

Atopy is a syndrome characterized by genetic tendency to develop allergic diseases, such as asthma, allergic rhinitis and atopic dermatitis. The risk factors of atopic diseases can be placed in two categories, namely host and environmental factors. The host factors of allergy include genetics, race, gender, age… etc. The environmental factors include exposure to environmental pollution and allergens. To define the distribution of atopic conditions (asthma, allergic rhinitis and atopic dermatitis) according to age, residence and available skin prick test. This cross-sectional study included (100) patients, representing research sample. It was conducted in specialized allergy center in Kirkuk city during the period from first January .2016 to the end of December 2016 All patients included in this study were referred from primary health centers, complaining from signs and symptoms of atopic diseases. Depending on medical history and clinical examinations, the sample was classified into (3) groups (asthma, allergic rhinitis and atopic dermatitis). Skin prick test was used to identify patients allergy to house dust mite and pollen. Regarding the distribution of samples according to residence, most patients (about 91%) were from urban areas compared with (9%) of them were from rural areas. Also, it was found that the frequency of atopic diseases decreases by age advancement. The frequency distribution of asthma, allergic rhinitis and atopic dermatitis was 49%, 26% and 25%, respectively. The skin test data revealed the frequency of 2 aeroallergen mite and pollen in all patients under study. .Hence, 65% were sensitive to house dust mite, 26% to pollen and 9% to both mite and pollen In conclusion this study confirmed that most patients complaining from atopic disease were from urban area. Also, the frequency of atopic diseases decreased by age advancement. The most common atopic disease was asthma followed by allergic rhinitis and atopic dermatitis. Most patients were sensitive to house dust mite.

Atopic dermatitis, house-dust mite, and the placebo effect

Allergy ◽  
2001 ◽  
Vol 56 (12) ◽  
pp. 1226-1226 ◽  
Author(s):  
C. Gutgesell ◽  
S. Heise ◽  
S. Seubert ◽  
A. Seubert ◽  
S. Domhof ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Placebo Effect ◽  
House Dust ◽  
House Dust Mite ◽  
Dust Mite

Epicutaneous application of house dust mite induces thymic stromal lymphopoietin in nonlesional skin of patients with atopic dermatitis

2013 ◽  
Vol 132 (5) ◽  
pp. 1252-1254 ◽  
Author(s):  
Janneke Landheer ◽  
Barbara Giovannone ◽  
Jeanine D. Mattson ◽  
Sandra Tjabringa ◽  
Carla A.F.M. Bruijnzeel-Koomen ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Thymic Stromal Lymphopoietin ◽  
Dust Mite

scholarly journals House dust mite allergens Der f and Der p induce IL-31 production by blood-derived T cells from atopic dermatitis patients

2017 ◽  
Vol 27 (4) ◽  
pp. 393-395 ◽  
Author(s):  
Krisztina Szegedi ◽  
Amanda van Lier ◽  
Pieter C. Res ◽  
Saskia Chielie ◽  
Jan D. Bos ◽  
...  
Keyword(s):  
T Cells ◽  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Dust Mite

Effect of House Dust Mite (HDM) Avoidance Measure on The Clinical Symptoms in Dogs with Atopic Dermatitis (AD)

2007 ◽  
Vol 119 (1) ◽  
pp. S281
Author(s):  
S. Kawarai ◽  
H. Shirai ◽  
M. Sakaguchi ◽  
K. Ohmori ◽  
N. Yasuda ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Clinical Symptoms ◽  
Dust Mite

scholarly journals The Role of Radioallergosorbent Testing (RAST) Early in the Management of Atopic Dermatitis in Children and Adults Referred to Secondary Care in the United Kingdom

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Secondary Care ◽  
High Serum ◽  
Cow’S Milk ◽  
Clinical Test ◽  
Cow's Milk ◽  
The United Kingdom ◽  
Dust Mite

Aim: To evaluate the importance and relevance of doing radioallegrosorbent testing (RAST) for patients referred with atopic dermatitis (AD) to secondary care for specialist dermatological advice and investigation. Background: Atopic dermatitis is usually diagnosed by Primary Care General Practitioners and treated using a stepped approach. Referral to secondary care is considered when it is not controlled with optimal treatment or if an allergy is suspected. Radioallegrosorbent testing is useful for identifying trigger factors however current literature lacks evidence for the extent to which RAST testing can improve patient symptoms. Method: Standard RAST testing was requested for all patients referred to secondary care with atopic dermatitis. Allergens tested included: dairy products, house dust mite, grass, nuts, seafood, cereals, dog dander and cat epithelium. Results: RAST results showed that 92.3% of children and 60.7% of adults had high total lgE levels. The proportion of children with high serum specific lgE for the allergens tested were as follows: house dust mite (60.4%), grass (50%), dairy/cow’s milk (39.5%), nuts (29.1%), dog dander (16.6%), cat epithelium (12.5%), egg (10.4%), fish/seafood (6.2%), wheat (6.2%), and soya (2.0%). The results for the adults tested were as follows: house dust mite (64.7%), grass (58.8%), cat epithelium (29%), dog dander (23%), nuts (11.7%), egg (11.7%), cow’s milk/dairy (5.8%), wheat/ soya (0%). On follow-up, all patients reported an improvement in their AD after implementing allergen avoidance strategies. Conclusions: RAST testing is a valuable clinical test that should be considered early to investigate patients with AD for relevant allergens that may be exacerbating their symptoms and causing resistance to first line treatment.

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