Effectiveness of Topical Application of Heterologous Platelet Rich Plasma (PRP) in Oral Mucous Membrane Pemphigoid. A Report of a Case

Mucous membrane pemphigoid (MMP) is a rare, predominantly mucosal subepithelialblistering disorder triggered by autoantibody reactivity to several basement membrane antigensincluding BP180, BP230, laminin 332, and type VII collagen [...]

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Oral Mucous Membrane Pemphigoid With Positive Nikolsky’s Sign: A Case Report

ACTA MEDICA IRANICA ◽

10.18502/acta.v58i12.5158 ◽

2021 ◽

Author(s):

Narjes Akbari ◽

Ghazaleh Mozafari ◽

Hamid Abbaszadeh

Keyword(s):

Case Report ◽

Basement Membrane ◽

Mucous Membrane ◽

Lateral Pressure ◽

Pemphigus Vulgaris ◽

Therapeutic Interventions ◽

Mucous Membrane Pemphigoid ◽

Oral Lesions ◽

Oral Mucous Membrane ◽

Gingival Mucosa

Mucous membrane pemphigoid (MMP) is a rare inflammatory, autoimmune, and subepithelial vesiculobullous disease in which tissue-bound autoantibodies are produced against one or more components of the basement membrane. Oral lesions of the pemphigoid begin in the form of vesicles or bullae that often involve throughout the mouth but may be confined to specific areas, especially the gingiva, in a pattern known as desquamative gingivitis. The positive Nikolsky's sign is characteristic of pemphigus vulgaris, in which a blister can appear on the normal-appearing skin if exerting lateral pressure, and is very rare in the mucosa and other vesiculobullous diseases. Here we report a case of mucous membrane pemphigoid that developed as desquamated gingivitis in a 46-year-old woman with positive Nikolsky's sign in the gingival mucosa. In the histopathologic view, a subepithelial cleft was observed. The results of direct and indirect immunofluorescence tests and related therapeutic interventions are also presented. Positive Nikolsky's sign can be observed in the mucosa as well as in the mucous membrane pemphigoid in addition to pemphigus vulgaris, and vesiculobullous lesions should be diagnosed based on the sum of clinical, histopathological, and immunofluorescence findings.

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A systematic review with pooled analysis of clinical presentation and immunodiagnostic testing in mucous membrane pemphigoid: association of anti-laminin-332 IgG with oropharyngeal involvement and the usefulness of ELISA

Journal of the European Academy of Dermatology and Venereology ◽

10.1111/jdv.13397 ◽

2015 ◽

Vol 30 (1) ◽

pp. 72-77 ◽

Cited By ~ 19

Author(s):

K.T. Amber ◽

R. Bloom ◽

M. Hertl

Keyword(s):

Systematic Review ◽

Mucous Membrane ◽

Clinical Presentation ◽

Pooled Analysis ◽

Mucous Membrane Pemphigoid ◽

Laminin 332

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The Alteration of the Epidermal Basement Membrane Complex of Human Nevus Tissue and Keratinocyte Attachment after High Hydrostatic Pressurization

BioMed Research International ◽

10.1155/2016/1320909 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Naoki Morimoto ◽

Chizuru Jinno ◽

Atsushi Mahara ◽

Michiharu Sakamoto ◽

Natsuko Kakudo ◽

...

Keyword(s):

Basement Membrane ◽

Immunohistochemical Staining ◽

Human Keratinocytes ◽

Membrane Complex ◽

Type Vii Collagen ◽

Transmission Electron ◽

Skin Reconstruction ◽

The Difference ◽

Laminin 332 ◽

Epidermal Basement Membrane

We previously reported that human nevus tissue was inactivated after high hydrostatic pressure (HHP) higher than 200 MPa and that human cultured epidermis (hCE) engrafted on the pressurized nevus at 200 MPa but not at 1000 MPa. In this study, we explore the changes to the epidermal basement membrane in detail and elucidate the cause of the difference in hCE engraftment. Nevus specimens of 8 mm in diameter were divided into five groups (control and 100, 200, 500, and 1000 MPa). Immediately after HHP, immunohistochemical staining was performed to detect the presence of laminin-332 and type VII collagen, and the specimens were observed by transmission electron microscopy (TEM). hCE was placed on the pressurized nevus specimens in the 200, 500, and 1000 MPa groups and implanted into the subcutis of nude mice; the specimens were harvested at 14 days after implantation. Then, human keratinocytes were seeded on the pressurized nevus and the attachment was evaluated. The immunohistochemical staining results revealed that the control and 100 MPa, 200 MPa, and 500 MPa groups were positive for type VII collagen and laminin-332 immediately after HHP. TEM showed that, in all of the groups, the lamina densa existed; however, anchoring fibrils were not clearly observed in the 500 or 1000 MPa groups. Although the hCE took in the 200 and 500 MPa groups, keratinocyte attachment was only confirmed in the 200 MPa group. This result indicates that HHP at 200 MPa is preferable for inactivating nevus tissue to allow its reuse for skin reconstruction in the clinical setting.

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