Application of the phenol-sulfuric acid method for the determination of glycogen in skeletal muscles and liver of rats

2021 ◽  
Author(s):  
A.M. Tretyakova ◽  
N.A. Vakhnina
Keyword(s):  
Sulfuric Acid ◽  
Key Words ◽  
Skeletal Muscles ◽  
Acid Method ◽  
Sulfuric Acid Method ◽  
Phenol Sulfuric Acid

The possibility of using the phenol-sulfuric acid method for the determination of total glycogen, its acid-soluble and acid-insoluble fractions in the liver and skeletal muscles of rats was studied. It was found that the use of a precipitant in the isolation of total glycogen and its fractions increases the yield of the investigated substances. Key words: phenol-sulfate method, rats, liver, muscles, total glycogen, acid-soluble glycogen, acid-insoluble glycogen.

An improved phenol-sulfuric acid method for the determination of carbohydrates in the presence of persulfate

2020 ◽  
Vol 227 ◽  
pp. 115332 ◽  
Author(s):  
Wen-Hui Zhang ◽  
Jian Wu ◽  
Liangyu Weng ◽  
Hongjie Zhang ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Acid Method ◽  
Sulfuric Acid Method ◽  
Phenol Sulfuric Acid

scholarly journals Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method

2010 ◽  
Vol 62 (3) ◽  
pp. 669-676 ◽  
Author(s):  
Xionggang Xi ◽  
Xinlin Wei ◽  
Yuanfeng Wang ◽  
Qinjie Chu ◽  
Jianbo Xiao
Keyword(s):  
Sulfuric Acid ◽  
Camellia Sinensis ◽  
Incubation Temperature ◽  
Experimental Conditions ◽  
Acid Method ◽  
Relative Standard ◽  
Sulfuric Acid Method ◽  
Concentrated Sulfuric Acid ◽  
Phenol Sulfuric Acid

A direct procedure for the determination of total polysaccharides (TPS) in Camellia sinensis was set up based on the modified phenol-sulfuric acid method. The monosaccharide composition of TPS was analyzed by GC. Based on the results of GC, model monosaccharide mixtures were made which provided an adequate standard for this procedure. Through single-factor and orthogonal (L934) experiments, the experimental conditions such as the volume of phenol, the volume of concentrated sulfuric acid, the reaction time, and the incubation temperature, were optimized. The highest sensitivity of absorbance was obtained when the volume of concentrated sulfuric acid, the volume of phenol (6%), and the incubation temperature were 2.5 ml, 0.2 ml, and 50?C, respectively. Under optimum conditions, the prepared samples were determined satisfactorily, with the recovery from 100.2% to 103.7%, and a relative standard deviation (RSD) of 2.1%. Overall, the modified method is easily operated, rapid, sensitive and accurate. A similar procedure can be applied to the determination of other plant polysaccharides as well. .

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