Recovery of used acetic acid via sulfuric acid

Kazakhstan has a huge phosphate raw material base, where the basis is made up of micro-grained phosphate ores of the Karatau basin. The depletion of reserves of high-quality commercial ores leads to the search for new methods of using the enrichment and sorting of low-grade technogenic ores, one of which is phosphate-siliceous slates. The presented study was carried out in two stages: at the first stage, phosphate-siliceous shales were enriched by the acetic acid method, regime technological parameters, kinetic and thermodynamic regularities of the process were determined. At the second stage of the research a method for recycling used acetic acid during the enrichment of low-grade phosphate-siliceous slates is proposed. In this case, sulfuric acid was chosen as the regenerating agent of acetic acid. The reliability of the performed studies was proved by the use of modern complex research methods: scanning electron microscopy, Energy Dispersive X-Ray and X-Ray difraction analyses. To determine the course of a particular reaction, a thermodynamic analysis was performed using modern HSC 6.0 software. The kinetic data are determined by calculation. The obtained experimental data were subjected to statistical analysis (Chaddock scale, standard deviation, coefficient of determination). The mechanism of interaction of an acetate solution with sulfuric acid is illustratively described. In conclusion, the sulfuric acid method is suitable for the regeneration of applied acetic acid. In this case, a by-product is formed in the form of calcium sulfate. This product can be used as a building binder (confirmed by the protocol of the “National Center of Expertise” of the Republic of Kazakhstan).

DETEKSI PRE-EKLAMPSIA PADA IBU HAMIL TRIMESTER 2-3 MELALUI PEMERIKSAAN PROTEIN URIN

Pre-eclampsia is a pregnancy-specific condition characterized by systemic dysfunction with endothelial activation and coagulation. This condition is characterized by blood pressure 140/90 mmHg after 20 weeks of gestation or sometimes earlier, accompanied by proteinuria ≥ 300 mg/24 hours and/or edema. This research is descriptive observational using 6% acetic acid method. The number of respondents in this study were 30 urine samples of pregnant women. The results of the examination are known from a total of 30 pregnant women respondents, as many as 15 proteinuria was detected. The most proteinuria in pregnant women is positive +/1 with the highest number in the age range of 30 -35 years. Proteinuria is most detected in the third trimester of pregnancy and occurs in the group of working pregnant women.

Application of the phenol-sulfuric acid method for the determination of glycogen in skeletal muscles and liver of rats

The possibility of using the phenol-sulfuric acid method for the determination of total glycogen, its acid-soluble and acid-insoluble fractions in the liver and skeletal muscles of rats was studied. It was found that the use of a precipitant in the isolation of total glycogen and its fractions increases the yield of the investigated substances. Key words: phenol-sulfate method, rats, liver, muscles, total glycogen, acid-soluble glycogen, acid-insoluble glycogen.

Quantification of total sugars and reducing sugars of dragon fruit-derived sugar-samples by UV-Vis spectrophotometric method

In the present work, the phenol-sulfuric acid method and the 3,5-dinitrosalicylic acid (DNS) method were developed with the aim to quantitatively analyze total sugars and reducing sugars, respectively. In regard with the phenol-sulfuric acid assay, 1.0 mL of sample was treated with 1.0 mL of 5% phenol, 5.0 mL of concentrated H2SO4 and measured at 485 nm, with the linearity range of 10–100 ppm for total sugars. The DNS method was performed on 2.0 mL of sample, using 1.5 mL of DNS at 80 °C for 10 minutes and measured at 510 nm, with the linearity range of 50–400 ppm for reducing sugars. The sugar contents of white dragon fruit-derived sugar-samples (extracted from species in Binh Thuan province, Vietnam) were also estimated by the above measured methods, exhibiting the total sugars of above 90% and the reducing sugars of above 5%. The methods were well-performed with the acceptable relative standard deviations of repeatability in accordance with the Association of Official Analytical Chemists (AOAC).

Edamame soybean protein concentrate as a source of amino acid nutrition for poultry

This study aims to determine the opportunity and characteristics of edamame soybean protein concentrate (eSPC) as a source of amino acid. In this study, edamame soybean protein concentrate (eSPC) was manufactured using various methods of manufacturing solvent-based materials. This experiment consisted of two different methods, namely washing with acid and ethanol solvent. The results of manufacturing eSPC were analyzed for nutrients, especially the protein and amino acids contents. From the presentation and description of data, the best results of edamame soybean processing into protein concentrate (eSPC) was obtained in the acid method with a protein content of 48.83% and a total of 36.66% amino acids. The highest content of lysine was obtained at eSPC processing using 3.77% of acid, while methionine highest was with approximately 1.12%. These results showed that the eSPC is well used as a source of lysine and methionine in poultry feed.

Study of serum malondialdehyde and uric acid levels in patients with malaria

Malaria is parasitic disease of humans caused by parasitic protozoan and genus plasmodium, widely present in tropical region. In the blood, the parasite travel to the liver to mature and reproduce. Oxidative stress is generated through the invasion of malarial parasites in human system. Malondialdehyde is a highly reactive compound is assayed in vivo as a biomarker of oxidative stress. Uric acid contributes to the pathology of human malaria by stimulating the production of cytokines from immune system. To estimate serum MDA & serum uric acid levels in patients with malarial infection and compare same with healthy individuals. This is a cross-sectional observational study, cases and controls were selected using random sampling method, attending hospital OPD. Study includes 50 laboratory diagnosed cases of malaria patients with equal age and sex matched controls. MDA was estimated using MDA - thiobarbituric acid method, uric acid was estimated by phosphotungstic acid method. Standardization of both the methods was carried out prior to experiment. There is generalized increase in serum MDA and uric acid levels in cases as compared to the control group.

Research on Purification Technology of Ultra-Large Flake Graphite Based on Alkali-Acid Method

Graphite is a strategically scarce resource, and the preparation of high-purity graphite is the prerequisite and basis for the application of graphite. In order to determine the optimal purification technology parameters of an ultra-large flake graphite mine pneumatic separation ore with a fixed carbon content of 77.69%, a particle size of mainly 10 to 40 mesh, and main impurities of calcium carbonate, iron oxide and silica , two additional experiments of acid method and alkali method were added on the basis of alkali-acid method, to investigate the purification effect of different technological processes and acid leaching times on graphite raw materials, as well as to analyze the retention extent of different methods and alkali fusion temperature on graphite ultra-large flake structure. The results show that all three methods can increase the fixed carbon content of graphite to above 99%. However, compared with the acid method and the alkali method, the alkali-acid method can obtain high-purity graphite while also better protecting the graphite's ultra-large flake structure. The optimal fusion temperature is 400 °C, the optimal acid leaching time is 30% sulfuric acid thrice and 5% hydrofluoric acid once. After purification, the fixed carbon content of the product exceeds 99.97%.

Fish Bone Gelatin

The purpose of this review article is to examine the method of making gelatin, the characteristics of gelatin from the results of research that has been carried out in Indonesia and the benefits of fish gelatin. Based on a review of various articles and other literature, it can be concluded that fish bone gelatin can be extracted by the acid method. The production of fishbone gelatin consists of 4 stages, the preparation of raw materials includes removal of non-collagen components from raw materials, conversion of collagen to gelatin, purification of gelatin by filtering and finally drying and powdering. Fishbone gelatin can be applied to both the food and non-food industries.