Faculty Opinions recommendation of Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.

2002 ◽  
Author(s):  
Viki Allan
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Envelope Breakdown

scholarly journals Nuclear Envelope Breakdown Proceeds by Microtubule-Induced Tearing of the Lamina

Cell ◽  
2002 ◽  
Vol 108 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Joël Beaudouin ◽  
Daniel Gerlich ◽  
Nathalie Daigle ◽  
Roland Eils ◽  
Jan Ellenberg
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Envelope Breakdown

Herpesvirus nuclear egress: Pseudorabies Virus can simultaneously induce nuclear envelope breakdown and exit the nucleus via the envelopment–deenvelopment-pathway

2015 ◽  
Vol 209 ◽  
pp. 76-86 ◽  
Author(s):  
Katharina S. Schulz ◽  
Barbara G. Klupp ◽  
Harald Granzow ◽  
Lars Paßvogel ◽  
Thomas C. Mettenleiter
Keyword(s):  
Nuclear Envelope ◽  
Pseudorabies Virus ◽  
Nuclear Envelope Breakdown ◽  
Nuclear Egress

Nuclear envelope removal/maintenance determines the structural and functional remodelling of embryonic red blood cell nuclei in activated mouse oocytes

Zygote ◽  
1998 ◽  
Vol 6 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Daniel Szöllösi ◽  
Renata Czołowska ◽  
Ewa Borsuk ◽  
Maria S. Szöllösi ◽  
Pascale Debey
Keyword(s):  
Nuclear Envelope ◽  
Transcriptional Activity ◽  
Chromatin Condensation ◽  
Oocyte Activation ◽  
Cell Nuclei ◽  
Nuclear Envelope Breakdown ◽  
Mouse Oocytes ◽  
Female Pronucleus ◽  
Mouse Fetuses

SummaryNuclei of embryonic red blood cells (e-RBC) from 12-day mouse fetuses are arrested in Go phase of the cell cycle and have low transcriptional activity. These nuclei were transferred with help of polyethylene glycol (PEG)-mediated fusion to parthenogenetically activated mouse oocytes and heterokaryons were analysed for nuclear structure and transcriptional activity. If fusion proceeded 25–45 min after oocyte activation, e-RBC nuclei were induced to nuclear envelope breakdown and partial chromatin condensation, followed by formation of nuclei structurally identical with pronuclei. These ‘pronuclei’, similar to egg (female) pronuclei, remained transcriptionally silent over several hours of in vitro culture. If fusion was performed 1 h or later (up to 7 h) after activation, the nuclear envelope of e-RBC nuclei remained intact and nuclear remodelling was less spectacular (slight chromatin decondensation, formation of nucleolus precursor bodies). These nuclei, however, reinforced polymerase-II-dependent transcription within a few hours of in vitro culture. Our present experiments, together with our previous work, demonstrate that nuclear envelope breakdown/maintenance are critical events for nuclear remodelling in activated mouse oocytes and that somatic dormant nuclei can be stimulated to renew transcription at a time when the female pronucleus remains transcriptionally silent.

scholarly journals Redistribution of centrosomal proteins by centromeres and Polo kinase controls nuclear envelope breakdown

2020 ◽  
Author(s):  
Andrew J. Bestul ◽  
Zulin Yu ◽  
Jay R. Unruh ◽  
Sue L. Jaspersen
Keyword(s):  
Nuclear Envelope ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Ring Structure ◽  
Yeast Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spindle Formation ◽  
Nuclear Envelope Breakdown ◽  
Polo Kinase

AbstractProper mitotic progression in Schizosaccharomyces pombe requires partial nuclear envelope breakdown (NEBD) and insertion of the spindle pole body (SPB – yeast centrosome) to build the mitotic spindle. Linkage of the centromere to the SPB is vital to this process, but why that linkage is important is not well understood. Utilizing high-resolution structured illumination microscopy (SIM), we show that the conserved SUNprotein Sad1 and other SPB proteins redistribute during mitosis to form a ring complex around SPBs, which is a precursor for NEBD and spindle formation. Although the Polo kinase Plo1 is not necessary for Sad1 redistribution, it localizes to the SPB region connected to the centromere, and its activity is vital for SPB ring protein redistribution and for complete NEBD to allow for SPB insertion. Our results lead to a model in which centromere linkage to the SPB drives redistribution of Sad1 and Plo1 activation that in turn facilitate NEBD and spindle formation through building of an SPB ring structure.SummaryNuclear envelope breakdown is necessary for fission yeast cells to go through mitosis. Bestul et al. show that the SUN protein, Sad1, is vital in carrying out this breakdown and is regulated by the centromere and Polo kinase.

An In Vitro System to Study Nuclear Envelope Breakdown

2014 ◽  
pp. 255-276 ◽  
Author(s):  
Joseph Marino ◽  
Lysie Champion ◽  
Cornelia Wandke ◽  
Peter Horvath ◽  
Monika I. Mayr ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
In Vitro System ◽  
Nuclear Envelope Breakdown
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