Faculty Opinions recommendation of Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells.

2006 ◽  
Author(s):  
Geneviève Dupont
Keyword(s):  
Intracellular Calcium ◽  
Living Cells ◽  
Calcium Oscillations ◽  
Inositol 1,4,5 Trisphosphate

scholarly journals Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells

2006 ◽  
Vol 173 (5) ◽  
pp. 755-765 ◽  
Author(s):  
Toru Matsu-ura ◽  
Takayuki Michikawa ◽  
Takafumi Inoue ◽  
Atsushi Miyawaki ◽  
Manabu Yoshida ◽  
...  
Keyword(s):  
Metabotropic Glutamate Receptor ◽  
Living Cells ◽  
Calcium Oscillations ◽  
Spike Generation ◽  
Metabotropic Glutamate ◽  
Endogenous Histamine ◽  
Rate Of Increase ◽  
Constant Rate ◽  
Inositol 1,4,5 Trisphosphate ◽  
Stimulation Of

We developed genetically encoded fluorescent inositol 1,4,5-trisphosphate (IP3) sensors that do not severely interfere with intracellular Ca2+ dynamics and used them to monitor the spatiotemporal dynamics of both cytosolic IP3 and Ca2+ in single HeLa cells after stimulation of exogenously expressed metabotropic glutamate receptor 5a or endogenous histamine receptors. IP3 started to increase at a relatively constant rate before the pacemaker Ca2+ rise, and the subsequent abrupt Ca2+ rise was not accompanied by any acceleration in the rate of increase in IP3. Cytosolic [IP3] did not return to its basal level during the intervals between Ca2+ spikes, and IP3 gradually accumulated in the cytosol with a little or no fluctuations during cytosolic Ca2+ oscillations. These results indicate that the Ca2+-induced regenerative IP3 production is not a driving force of the upstroke of Ca2+ spikes and that the apparent IP3 sensitivity for Ca2+ spike generation progressively decreases during Ca2+ oscillations.

scholarly journals Inositol (1,4,5)-Trisphosphate Dynamics and Intracellular Calcium Oscillations in Pancreatic  -Cells

Diabetes ◽  
2005 ◽  
Vol 54 (11) ◽  
pp. 3073-3081 ◽  
Author(s):  
N. A. Tamarina ◽  
A. Kuznetsov ◽  
C. J. Rhodes ◽  
V. P. Bindokas ◽  
L. H. Philipson
Keyword(s):  
Intracellular Calcium ◽  
Calcium Oscillations ◽  
Pancreatic Cells ◽  
Inositol 1,4,5 Trisphosphate

scholarly journals Intracellular calcium channels: Inositol-1,4,5-trisphosphate receptors

2014 ◽  
Vol 739 ◽  
pp. 39-48 ◽  
Author(s):  
Olena A. Fedorenko ◽  
Elena Popugaeva ◽  
Masahiro Enomoto ◽  
Peter B. Stathopulos ◽  
Mitsuhiko Ikura ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Intracellular Calcium ◽  
Inositol 1,4,5 Trisphosphate

scholarly journals Inositol 1,4,5 trisphosphate releases calcium from specialized sites within Limulus photoreceptors

1987 ◽  
Vol 104 (4) ◽  
pp. 933-937 ◽  
Author(s):  
R Payne ◽  
A Fein
Keyword(s):  
Endoplasmic Reticulum ◽  
Injection Site ◽  
Intracellular Calcium ◽  
Subcellular Distribution ◽  
Smooth Endoplasmic Reticulum ◽  
Image Intensifier ◽  
Photoreceptor Cells ◽  
Calcium Stores ◽  
Inositol 1,4,5 Trisphosphate ◽  
Probable Site

We have investigated the subcellular distribution and identity of inositol trisphosphate (InsP3)-sensitive calcium stores in living Limulus ventral photoreceptor cells, where light and InsP3 are known to raise intracellular calcium. We injected ventral photoreceptor cells with the photoprotein aequorin and viewed its luminescence with an image intensifier. InsP3 only elicited detectable aequorin luminescence when injected into the light-sensitive rhabdomeral (R)-lobe where aequorin luminescence induced by light was also confined. Calcium stores released by light and InsP3 are therefore localized to the R-lobe. Within the R-lobe, InsP3-induced aequorin luminescence was further confined around the injection site, due to rapid dilution and/or degradation of injected InsP3. Prominent cisternae of smooth endoplasmic reticulum are uniquely localized within the cell beneath the microvillar surface of the R-lobe (Calman, B., and S. Chamberlain, 1982, J. Gen. Physiol., 80:839-862). These cisternae are the probable site of InsP3 action.

The existence of inositol 1,4,5-trisphosphate and ryanodine receptors in mature bovine oocytes

Development ◽  
1995 ◽  
Vol 121 (8) ◽  
pp. 2645-2654 ◽  
Author(s):  
C. Yue ◽  
K.L. White ◽  
W.A. Reed ◽  
T.D. Bunch
Keyword(s):  
Ryanodine Receptors ◽  
Inhibitory Effect ◽  
Ruthenium Red ◽  
Calcium Oscillations ◽  
Ip3 Receptor ◽  
Cyclic Adp Ribose ◽  
Inositol 1,4,5 Trisphosphate ◽  
Bovine Oocytes ◽  
Continuous Injection

Intracellular Ca2+ (Ca2+i) transients during fertilization are critical to the activation of eggs in all species studied. Activation of both the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RYR) are responsible for the calcium oscillations during fertilization in sea urchin eggs. Using in vitro matured bovine oocytes loaded with Fura-2 AM ester as Ca2+i indicator, we addressed whether IP3Rs and RYRs coexist in mammalian eggs. Our results indicate that microinjection of 50–250 nM IP3 or 10–20 mM caffeine, 100–200 microM ryanodine and 4–8 microM cyclic ADP-ribose all induced Ca2+i release. The Ca2+i release induced by 250 nM IP3 could only be inhibited by prior injection of 1 mg/ml heparin which was overcome by continuous injection of IP3 to 1 microM. Prior injection of either 50 microM ruthenium red, 50 microM procaine or 1 % vehicle medium (VM) did not affect the Ca2+i release induced by IP3. Prior injection of heparin or VM did not affect the Ca2+i release induced by 10–20 mM caffeine or 200 microM ryanodine, but prior injection of 50 microM ruthenium red or procaine completely inhibited the effect of 10–20 mM caffeine. In addition, continuous injection of caffeine up to 40 mM overcame the inhibitory effect of ruthenium red or procaine. The same 50 microM concentration of ruthenium red or procaine only partially blocked the effect of 200 microM ryanodine, but 200 microM ruthenium red or procaine completely blocked the effect of 200 microM ryanodine.(ABSTRACT TRUNCATED AT 250 WORDS)

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