Faculty Opinions recommendation of Reverse biological engineering of hrdB to enhance the production of avermectins in an industrial strain of Streptomyces avermitilis.

2010 ◽  
Author(s):  
Malcolm Whiteway
Keyword(s):  
Streptomyces Avermitilis ◽  
Biological Engineering ◽  
Industrial Strain

Construction of a doramectin producer mutant from an avermectin-overproducing industrial strain of Streptomyces avermitilis

2009 ◽  
Vol 55 (12) ◽  
pp. 1355-1363 ◽  
Author(s):  
Xuejin Zhao ◽  
Yuanxin Wang ◽  
Shiwei Wang ◽  
Zhi Chen ◽  
Ying Wen ◽  
...  
Keyword(s):  
Deletion Mutant ◽  
Fermentation Medium ◽  
Streptomyces Avermitilis ◽  
Industrial Strain ◽  
Agricultural Pesticides ◽  
Double Deletion ◽  
Resistance Markers ◽  
Encoding Gene ◽  
Branched Chain ◽  
Encoding Genes

The avermectin analogue doramectin (CHC-B1), which is produced in mutants that have an altered biosynthesis pathway of avermectin, is one of the most effective agricultural pesticides and antiparasitics. We report here the construction of a bkdF olmA double-deletion mutant lacking one of the branched-chain α-keto acid dehydrogenase encoding genes (bkdF) and the oligomycin PKS encoding gene cluster (olmA) in Streptomyces avermitilis 76-05. We then characterized the production of various antibiotics in cultures of the deletion mutant. In a fermentation medium supplemented with cyclohexanecarboxylic acid, this double mutant produced doramectin and its analogues but no oligomycin. The mutant proved to be genetically stable, without any antibiotic resistance markers inserted into its chromosome, and could potentially become an industrial doramectin-producing strain after further improvement.

scholarly journals Streptomyces avermitilis industrial strain as cell factory for Ivermectin B1a production

2019 ◽  
Vol 4 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Qian Deng ◽  
Liqiongzi Xiao ◽  
Yang Liu ◽  
Lixin Zhang ◽  
Zixin Deng ◽  
...  
Keyword(s):  
Streptomyces Avermitilis ◽  
Cell Factory ◽  
Industrial Strain

Corrigendum: Construction of a doramectin producer mutant from an avermectin-overproducing industrial strain of Streptomyces avermitilis

2010 ◽  
Vol 56 (1) ◽  
pp. 88-88
Author(s):  
Xuejin Zhao ◽  
Yuanxin Wang ◽  
Shiwei Wang ◽  
Zhi Chen ◽  
Ying Wen ◽  
...  
Keyword(s):  
Streptomyces Avermitilis ◽  
Industrial Strain

Direct proteomic mapping of Streptomyces avermitilis wild and industrial strain and insights into avermectin production

2013 ◽  
Vol 79 ◽  
pp. 1-12 ◽  
Author(s):  
Peng Yin ◽  
You-Yuan Li ◽  
Jin Zhou ◽  
Yong-Hong Wang ◽  
Si-Liang Zhang ◽  
...  
Keyword(s):  
Streptomyces Avermitilis ◽  
Industrial Strain
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