Environmental Pyrethroid Exposure and Cognitive Dysfunction in U.S. Older Adults: The NHANES 2001–2002

Pyrethroid compounds are widely used in household insecticides and agricultural pesticides. Recent studies, however, report that pyrethroid exposures affect neurobehavioral function in animals and may be associated with adverse neurocognitive development in children. This study aimed to examine the association between pyrethroid exposure and cognitive dysfunction in older adults using a well-defined general population. We analyzed data from 336 individuals, aged 60–84 years, who participated in the National Health and Nutrition Examination Survey 2001–2002. We used urinary 3-phenoxybenzoic acid (3-PBA) concentration as a biomarker of pyrethroid exposures and assessed cognitive function with the digit–symbol coding test. The geometric means (±geometric standard errors) of creatinine-uncorrected and corrected urinary 3-PBA were 0.30 (±0.87) μg/L and 0.36 (±0.89) μg/g. After adjusting for sociodemographic factors, higher 3-PBA concentrations (> vs. ≤0.30 μg/g creatinine (median)) were associated with lower scores of cognitive function (−3.83 95% confidence interval: −7.11, −0.54). Significance was persistent after additionally adjusting for physical activity and smoking pack-year (−3.76 95% CI: −7.16, −0.36) and further adjusting for BMI and presence of hypertension and diabetes (−3.82 95% CI: −6.92, −0.71). Our findings suggest that pyrethroid exposure is associated with cognitive dysfunction in older adults.

Contamination methods of milk with pesticides residues and veterinary drugs

The use of agricultural pesticides is very important in improving production, but the residue of these pesticides on crops have a serious health effect on humans. On the other hand, there is another type of pollutants resulting from the use of veterinary medicines, as well as heavy metals and mycotoxins that also result in health risks to humans due to their consumption of milk and dairy products. Milk is an important and necessary food for the body as it is consumed daily by many people because its contain fat, protein, vitamin, and minerals. In this work, we present a review of most scientific articles from 1964 until 2020, related to evaluating milk contamination with various chemicals, especially pesticides and veterinary drugs. We observe from many research papers at different country in the world Lingering presence detectable values of chemical contaminate The maximum remainder, as a final result of this review paper we need to establish monitor program system for all chemical contaminate residue in milk and many food type to improve safety and reduce the risk for consumers in Iraq.

Short-term metabolic resistance inductive effect of different agrochemical groups on Anopheles gambiae mosquitoes

Background: In order to assess the impact of the different groups of agricultural pesticides used in Côte d'Ivoire on the increase of mosquitoes resistance to insecticides, the expression profiles of 7 P450 cytochromes and one GSTE2 of Anopheles gambiae involved in mosquito resistance to insecticides were studied. The goal of this study was to determine the effect of short exposure of mosquito larvae to different groups of agricultural pesticides on mosquito resistance. Methods: Three groups of pesticides were selected: (i) agricultural insecticide solutions, (ii) non-insecticide pesticide solutions (a mixture of herbicides and fungicides), and (iii) a mixture of the first two. A fourth non-pesticide solution was used as a control. Four groups of each stage 2 larvae (strain Kisumu, male and female) were exposed to 20% concentrated solution for 24 hours. Susceptibility tests for dichlorodiphenyltrichloroethane (DDT) and Deltamethrin were carried out on adults aged 2-5 days. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed to quantify the expression of eight metabolic genes involved in mosquito resistance to insecticides. Results: Susceptibility to DDT showed a similar increase in the time required to knock down 50% of mosquitoes (kdt50) in l colonies exposed to insecticides and non-insecticides compared to the control colony. As for deltamethrin, kdt50 was higher in the colonies exposed to insecticides and the pesticide mixture compared to the colony exposed to non-insecticides. Of all the genes studied in all colonies, except for CYP6P1 induced only in the colony consisting of the pesticide mixture, no genes were induced. Conclusions: This study confirmed that induction is influenced by the duration, the concentration of the solution and the type of xenobiotic used as an inducer. The overexpression of CYP6P1 confirmed the inductive effect that a short exposure of mosquito larvae to agricultural pesticides could have.

Sensitivity of The Stripe-Faced Dunnart, Sminthopsis Macroura (Gould 1845), To The Phenyl Pyrazole Insecticide, Fipronil, Toxicological Signs And Implications For Pesticide Risk Assessments In Australia

A lack of toxicity data quantifying responses of Australian native mammals to agricultural pesticides prompted an investigation into the sensitivity of the stripe-faced dunnart, Sminthopsis macroura (Gould 1845) to the insecticide, fipronil (5-amino-3-cyano-1-(2,6-dichloro-4-trifluoromethylphenyl)-4-trifluoromethylsulfinyl pyrazole, CAS No. 120068-37-3). Using the Up-And-Down method for determining acute oral toxicity in mammals, derived by the Organisation for Economic Cooperation and Development (OECD), median lethal dose estimates of 990 mg kg− 1 (95% confidence interval (CI) = 580.7–4770.0 mg kg− 1) and 270.4 mg kg− 1 (95% CI = 0.0 - >20000.0 mg kg− 1) were resolved for male and female S. macroura respectively. The difference between median lethal dose estimates for males and females may have been influenced by the increased age of two female dunnarts. Further modelling of female responses to fipronil doses used the following assumptions: (a) death at 2000 mg kg− 1, (b) survival at 500 mg kg− 1 and (c) a differential response (both survival and death) at 990 mg kg− 1. This modelling revealed median lethal dose estimates for female S. macroura of 669.1 mg kg− 1 (95% CI = 550–990 mg kg− 1; assuming death at 990 mg kg− 1) and 990 mg kg− 1 (95% CI = 544.7–1470 mg kg− 1; assuming survival at 990 mg kg− 1). These median lethal dose estimates are 3–10-fold higher than the only available LD50 value for a similarly sized eutherian mammal, Mus musculus (L. 1758; 94 mg kg− 1) and that available for Rattus norvegicus (Birkenhout 1769; 97 mg kg− 1). Implications for pesticide risk assessments in Australia are discussed.

A Method Scope Extension for the Simultaneous Analysis of POPs, Current-Use and Banned Pesticides, Rodenticides, and Pharmaceuticals in Liver. Application to Food Safety and Biomonitoring

The screening of hundreds of substances belonging to multiple chemical classes in liver is required in areas such as food safety or biomonitoring. We adapted a previous QuEChERS-based method in blood to the liver matrix and applied to these fields of study. The validation of the method allowed the inclusion of 351 contaminants, 80% with a LOQ < 2 ng/g. In the analysis of 42 consumer liver samples, we detected trace levels of 29 different contaminants. The most frequent and concentrated was 4,4’-DDE. POPs accounted for 66% of the compounds detected. In no case was the MRL reached for any of the contaminants detected. We also applied the method to 151 livers of wild birds to perform a biomonitoring pilot study in the Canary Islands. We detected 52 contaminants in 15 bird species. These were also mostly POPs, although high frequencies and concentrations of anticoagulant rodenticides (AR) and some other agricultural pesticides also stand out. POPs and AR contamination levels were significantly higher in terrestrial birds, raptors and particularly in nocturnal birds. Pesticide contamination levels were also higher in terrestrial birds, as well as in non-raptors and diurnal birds. The validated method is simple, robust, and sensitive and performs well in a variety of practical scenarios, where it can be carried out relatively quickly and inexpensively.

Entomopathogenic microorganisms: their role in insect pest management

Background Entomopathogens are pathogenic to insect pests. Several types of naturally occurring, viz. fungus, bacteria, viruses, and nematodes, infect a range of insect pests and help manage crop growth. They offer several advantages over chemical pesticides, including being precise, safe, and ecologically sustainable. Agricultural systems are streamlined, and changes to natural ecosystems occur. Viruses, bacteria are host-specific, while fungi have a greater host range, and they may infect both soil-dwelling and aboveground pests. Main body The study highlights the current state of knowledge on entomopathogenic microorganisms (EM) (entomopathogenic fungi, nematodes, viruses, bacteria, etc.) as it relates to their current usage as biological pest management. It is essential to enhance our understanding of the ecology of EM and their role in nature to use a variety of biological control techniques against insect hosts. This article may help to comprehend their accomplishments in the significant field. Some recent researches indicated common patterns in interactions between insect pests and EM. Conclusion More focus has been placed on the use of natural enemies like entomopathogens for pest control in recent years. EM expands possibilities for insect control. Eco-friendly alternatives to existing agricultural pesticides are being developed which are utilized to control insect pests and support agricultural sustainability.

1291Adapting a randomized trial design to develop epidemiology studies for pesticide exposure

Focus of Presentation Pesticides are chemicals, synthetic or natural, used to mitigate damage caused by pests. They play an important role in agriculture and public health. For a pesticide to be put on the market it must undergo a series of tests required by regulatory agencies to show with reasonable certainty that human exposures will not result in harm. Randomized trials are considered the gold standard for epidemiological study design. It is not ethical to conduct such studies to investigate human health outcomes of pesticide exposure. With increasing abundance of observational epidemiological literature there is an opportunity to leverage these studies for regulatory decision making. This presentation will describe a hypothetical randomized trial design to identify opportunities to adapt elements of experimental epidemiology to address some of the challenges unique to studies of health outcomes following pesticide exposure. Findings Elements warranting further development include analytic methods mimicking randomization, blinding, defining control groups, and introducing increased precision to outcome and exposure classifications. Efforts should aim to define generalizability and determine ways to report findings appropriate for regulatory use. Conclusions/Implications Epidemiologists must leverage analytic techniques, and increase dialogue among academics, industry stakeholders and regulators to adapt experimental epidemiology methods to ensure safety of agricultural pesticides. Key messages Work is needed to adapt experimental epidemiological methods to investigate human health outcomes of pesticide exposure. Focus areas for adaptation include randomization, blinding, control selection, exposure and outcome definitions, generalizability, and reporting.

8. International Regulation of Toxic and Persistent Pollutants and Waste

This chapter relates at toxic and persistent substances such as chemicals, plastics, industrial wastes, and agricultural pesticides to international environmental law. These substances certainly create an environmental risk which has an international dimension. The chapter outlines the reasons for this. First, the release of persistent and potentially toxic substances into the environment may have long-term and cumulative effects on human and animal health over a wide area, including the marine environment. Secondly, international trade in wastes and chemicals poses a potential risk of accidental pollution of the marine environment and of transit states. The chapter aims to illustrate the importance of adequate institutional machinery for supervising implementation of environmental protection treaties and ensuring their continued development.