Faculty Opinions recommendation of Microbial ecology and evolution: a ribosomal RNA approach.

Author(s):  
Jed Fuhrman
1986 ◽  
Vol 40 (1) ◽  
pp. 337-365 ◽  
Author(s):  
G J Olsen ◽  
D J Lane ◽  
S J Giovannoni ◽  
N R Pace ◽  
D A Stahl

Author(s):  
Daniel Beniac ◽  
George Harauz

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.


2008 ◽  
Vol 53 (1) ◽  
pp. 3-11 ◽  
Author(s):  
J Kuparinen ◽  
H Galvão
Keyword(s):  

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