Faculty Opinions recommendation of Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM.

2013 ◽  
Author(s):  
Hongwei Wang
Keyword(s):  
Single Particle ◽  
Motion Correction ◽  
Atomic Resolution ◽  
Electron Counting ◽  
Induced Motion ◽  
Resolution Single

scholarly journals Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

2013 ◽  
Vol 10 (6) ◽  
pp. 584-590 ◽  
Author(s):  
Xueming Li ◽  
Paul Mooney ◽  
Shawn Zheng ◽  
Christopher R Booth ◽  
Michael B Braunfeld ◽  
...  
Keyword(s):  
Single Particle ◽  
Motion Correction ◽  
Atomic Resolution ◽  
Electron Counting ◽  
Induced Motion ◽  
Resolution Single

A3Near-atomic Resolution Single Particle Analysis with the Volta Phase Plate

Microscopy ◽  
2015 ◽  
Vol 64 (suppl 1) ◽  
pp. i9.2-i9
Author(s):  
Radostin Danev ◽  
Maryam Khoshouei ◽  
Wolfgang Baumeister
Keyword(s):  
Single Particle ◽  
Atomic Resolution ◽  
Particle Analysis ◽  
Phase Plate ◽  
Single Particle Analysis ◽  
Resolution Single

scholarly journals A simple and robust procedure for preparing graphene-oxide cryo-EM grids

2018 ◽  
Author(s):  
Eugene Palovcak ◽  
Feng Wang ◽  
Shawn Q. Zheng ◽  
Zanlin Yu ◽  
Sam Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
High Resolution ◽  
Motion Correction ◽  
Biological Macromolecules ◽  
Robust Method ◽  
Partial Protection ◽  
Air Water Interface ◽  
Induced Motion ◽  
Resolution Single ◽  
Substrate Film

AbstractGraphene oxide (GO) sheets have been used successfully as a supporting substrate film in several recent cryogenic electron-microscopy (cryo-EM) studies of challenging biological macromolecules. However, difficulties in preparing GO-covered holey carbon EM grids have limited its widespread use. Here, we report a simple and robust method for covering holey carbon EM grids with GO sheets and demonstrate that these grids are suitable for high-resolution single particle cryo-EM. GO substrates adhere macromolecules, allowing cryo-EM grid preparation with lower specimen concentrations and providing partial protection from the air-water interface. Additionally, the signal from images of the GO lattice beneath the frozen-hydrated specimen can be discerned in many motion-corrected micrographs, providing a high-resolution fiducial for evaluating beam-induced motion correction.

scholarly journals Implementing Direct Electron Detection Camera K2 and Dose Fractionation for Near Atomic Resolution Single Particle CryoEM

2013 ◽  
Vol 19 (S2) ◽  
pp. 1152-1153 ◽  
Author(s):  
X. Li ◽  
S. Zheng ◽  
D.A. Agard ◽  
Y. Cheng
Keyword(s):  
Single Particle ◽  
Atomic Resolution ◽  
Dose Fractionation ◽  
Electron Detection ◽  
Resolution Single

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

scholarly journals Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

2021 ◽  
Vol 77 (a1) ◽  
pp. a209-a209
Author(s):  
Jonathan Bouvette ◽  
Mario Borgnia ◽  
Hsuan-Fu Liu ◽  
Alberto Bartesaghi ◽  
Roel Schaaper ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Single Particle ◽  
Electron Tomography ◽  
Atomic Resolution ◽  
Beam Image ◽  
Cryo Electron Tomography ◽  
Resolution Single

Rapid near-atomic resolution single-particle 3D reconstruction with SIMPLE

2018 ◽  
Vol 204 (2) ◽  
pp. 172-181 ◽  
Author(s):  
Cyril F. Reboul ◽  
Simon Kiesewetter ◽  
Michael Eager ◽  
Matthew Belousoff ◽  
Tiangang Cui ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Single Particle ◽  
Atomic Resolution ◽  
Resolution Single
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