Faculty Opinions recommendation of DNA polymerase beta participates in mitochondrial DNA repair.

2017 ◽  
Author(s):  
Aishwarya Prakash
Keyword(s):  
Dna Repair ◽  
Mitochondrial Dna ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Polymerase Beta ◽  
Mitochondrial Dna Repair

scholarly journals DNA Polymerase Beta Participates in Mitochondrial DNA Repair

2017 ◽  
Vol 37 (16) ◽  
Author(s):  
P. Sykora ◽  
S. Kanno ◽  
M. Akbari ◽  
T. Kulikowicz ◽  
B. A. Baptiste ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Polymerase ◽  
Excision Repair ◽  
Gene Knockout ◽  
Mitochondrial Protein ◽  
Human Kidney ◽  
Specific Protein ◽  
Dna Polymerase Beta ◽  
Mouse Tissue ◽  
Polymerase Beta

ABSTRACT We have detected DNA polymerase beta (Polβ), known as a key nuclear base excision repair (BER) protein, in mitochondrial protein extracts derived from mammalian tissue and cells. Manipulation of the N-terminal sequence affected the amount of Polβ in the mitochondria. Using Polβ fragments, mitochondrion-specific protein partners were identified, with the interactors functioning mainly in DNA maintenance and mitochondrial import. Of particular interest was the identification of the proteins TWINKLE, SSBP1, and TFAM, all of which are mitochondrion-specific DNA effectors and are known to function in the nucleoid. Polβ directly interacted functionally with the mitochondrial helicase TWINKLE. Human kidney cells with Polβ knockout (KO) had higher endogenous mitochondrial DNA (mtDNA) damage. Mitochondrial extracts derived from heterozygous Polβ mouse tissue and KO cells had lower nucleotide incorporation activity. Mouse-derived Polβ null fibroblasts had severely affected metabolic parameters. Indeed, gene knockout of Polβ caused mitochondrial dysfunction, including reduced membrane potential and mitochondrial content. We show that Polβ is a mitochondrial polymerase involved in mtDNA maintenance and is required for mitochondrial homeostasis.

An intrinsic 5′-deoxyribose-5-phosphate lyase activity in DNA polymerase beta from Leishmania infantum supports a role in DNA repair

DNA Repair ◽  
2006 ◽  
Vol 5 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Ana Alonso ◽  
Gloria Terrados ◽  
Angel J. Picher ◽  
Rafael Giraldo ◽  
Luis Blanco ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Leishmania Infantum ◽  
Dna Polymerase Beta ◽  
Polymerase Beta ◽  
Lyase Activity

scholarly journals Effects of DNA polymerase .BETA. gene over-expressed in transgenic Drosophila on DNA repair and recombination.

1994 ◽  
Vol 69 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Mi-Ae YOO ◽  
Won-Ho LEE ◽  
Hye-Yeong HA ◽  
Jae-Ryeon RYU ◽  
Masamitsu YAMAGUCHI ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Polymerase Beta ◽  
Transgenic Drosophila ◽  
Beta Gene

scholarly journals Chick embryo DNA polymerase beta. Purified enzyme consists of a single Mr = 40,000 polypeptide.

1980 ◽  
Vol 255 (20) ◽  
pp. 9942-9948 ◽  
Author(s):  
M. Yamaguchi ◽  
K. Tanabe ◽  
Y.N. Taguchi ◽  
M. Nishizawa ◽  
T. Takahashi ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Polymerase Beta
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