The Sample Preparation and the Improvement Method of Two-Dimensional Electrophoresis of Proteins from Antiaris toxicaria (Moraceae) Seed

2010 ◽  
Vol 31 (4) ◽  
pp. 357-362 ◽  
Author(s):  
Jun YANG ◽  
Yun-Qiang YANG ◽  
Yong-Ping YANG ◽  
Xiang-Yang HU
Keyword(s):  
Sample Preparation ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Improvement Method ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Sample preparation of human tumors prior to two-dimensional electrophoresis of proteins

1995 ◽  
Vol 16 (1) ◽  
pp. 1087-1089 ◽  
Author(s):  
Bo Franzén ◽  
Takashi Hirano ◽  
Ken Okuzawa ◽  
Kazuto Uryu ◽  
Ayodele A. Alaiya ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Tumors ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Analytical electrofocusing and two-dimensional electrophoresis of proteins extracted from the mycelia of aggressive and nonaggressive strains of Ophiostoma ulmi

1986 ◽  
Vol 64 (9) ◽  
pp. 2073-2081 ◽  
Author(s):  
Robert S. Jeng
Keyword(s):  
Dutch Elm Disease ◽  
Two Dimensional ◽  
Polyacrylamide Gels ◽  
Ophiostoma Ulmi ◽  
Two Dimensional Electrophoresis ◽  
Coomassie Blue ◽  
Isoelectric Points ◽  
Additional Protein ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Soluble mycelial proteins from Ophiostoma ulmi (Buism.) Nannf., the causal agent of Dutch elm disease, were separated by analytical electrofocusing and two-dimensional electrophoresis in polyacrylamide gels. Results showed the aggressive and nonaggressive strains of this pathogen each had about 60 Coomassie blue stained bands having isoelectric points from 3 to 7. Both strains of this fungus had their own characteristic electrofocusing patterns. Nonaggressive isolate S116, for example, lacked two protein bands, one near the anode and one near the cathode, but it had five additional protein bands distributed from pH 4 to 6. Two-dimensional electrophoresis of total soluble proteins depicted that there were 36 proteins found to be specific for the nonaggressive isolate S116 and 12 proteins for the aggressive isolate RR2.

Two-dimensional electrophoresis of proteins with a different approach to isoelectric focusing

The Analyst ◽  
1994 ◽  
Vol 119 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
S. M. Saqlan Naqvi ◽  
V. Cengiz Özalp ◽  
H. Avni Öktem ◽  
Meral Yücel
Keyword(s):  
Isoelectric Focusing ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins
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