scholarly journals Illumination Policies for Stichococcus sp. Cultures in an Optimally Operating Lab-Scale PBR toward the Directed Photosynthetic Production of Desired Products

2021 ◽  
Author(s):  
Paraskevi Psachoulia ◽  
Christos Chatzidoukas
Keyword(s):  
Photosynthetic Production

scholarly journals Over-expression of an electron transport protein OmcS provides sufficient NADH for d-lactate production in cyanobacterium

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hengkai Meng ◽  
Wei Zhang ◽  
Huawei Zhu ◽  
Fan Yang ◽  
Yanping Zhang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electron Transport ◽  
Lactate Production ◽  
Transport Protein ◽  
Dominant Form ◽  
Photosynthetic Production ◽  
Reducing Equivalent ◽  
Excess Electrons ◽  
Novel Strategy ◽  
Electron Transport Protein

Abstract Background An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equivalent. However, most dehydrogenases prefer to utilize NADH as a cofactor. Thus, sufficient NADH supply is crucial to produce dehydrogenase-derived chemicals in cyanobacteria. Photosynthetic electron is the sole energy source and excess electrons are wasted in the light reactions of photosynthesis. Results Here we propose a novel strategy to direct the electrons to generate more ATP from light reactions to provide sufficient NADH for lactate production. To this end, we introduced an electron transport protein-encoding gene omcS into cyanobacterium Synechococcus elongatus UTEX 2973 and demonstrated that the introduced OmcS directs excess electrons from plastoquinone (PQ) to photosystem I (PSI) to stimulate cyclic electron transfer (CET). As a result, an approximately 30% increased intracellular ATP, 60% increased intracellular NADH concentrations and up to 60% increased biomass production with fourfold increased d-lactate production were achieved. Comparative transcriptome analysis showed upregulation of proteins involved in linear electron transfer (LET), CET, and downregulation of proteins involved in respiratory electron transfer (RET), giving hints to understand the increased levels of ATP and NADH. Conclusions This strategy provides a novel orthologous way to improve photosynthesis via enhancing CET and supply sufficient NADH for the photosynthetic production of chemicals.

Photosynthetic Production of Ulva rotundata Bliding Estimated by Oxygen and Inorganic Carbon Exchange Measurements in the Field

2003 ◽  
Vol 46 (4) ◽  
Author(s):  
J. M. Mercado ◽  
A. Avilés ◽  
E. Benítez ◽  
M. Carrasco ◽  
L. Palomo ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Carbon Exchange ◽  
Photosynthetic Production

Photosynthetic Production of Ethanol Using Genetically Engineered Cyanobacteria

2020 ◽  
pp. 99-113
Author(s):  
F. P. De Andrade ◽  
M. L. F. De Sá Filho ◽  
R. R. L. Araújo ◽  
T. R. M. Ribeiro ◽  
A. E. Silva ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Photosynthetic Production

Large-scale photosynthetic production of carbon-13 labeled sugars: The tobacco leaf system

1973 ◽  
Vol 50 (3) ◽  
pp. 826-831 ◽  
Author(s):  
V.H. Kollman ◽  
J.L. Hanners ◽  
J.Y. Hutson ◽  
T.W. Whaley ◽  
D.G. Ott ◽  
...  
Keyword(s):  
Large Scale ◽  
Tobacco Leaf ◽  
Photosynthetic Production ◽  
Leaf System
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