Facile synthesis of a 3-deazaadenosine phosphoramidite for RNA solid-phase synthesis
2016 ◽
Vol 12
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pp. 2556-2562
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Keyword(s):
Access to 3-deazaadenosine (c3A) building blocks for RNA solid-phase synthesis represents a severe bottleneck in modern RNA research, in particular for atomic mutagenesis experiments to explore mechanistic aspects of ribozyme catalysis. Here, we report the 5-step synthesis of a c3A phosphoramidite from cost-affordable starting materials. The key reaction is a silyl-Hilbert–Johnson nucleosidation using unprotected 6-amino-3-deazapurine and benzoyl-protected 1-O-acetylribose. The novel path is superior to previously described syntheses in terms of efficacy and ease of laboratory handling.
2002 ◽
Vol 23
(11)
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pp. 1658-1660
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Keyword(s):
2000 ◽
Keyword(s):
Keyword(s):
2001 ◽
Vol 3
(1)
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pp. 34-44
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Keyword(s):
Keyword(s):
Keyword(s):