scholarly journals Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

10.3791/2160 ◽  
2010 ◽  
Author(s):  
Steve P. Hawley ◽  
Melanie K. B. Wills ◽  
Nina Jones
Keyword(s):  
Signaling Molecules ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Primary Mouse

scholarly journals Gene Targeting of Cdc42 and Cdc42GAP Affirms the Critical Involvement of Cdc42 in Filopodia Induction, Directed Migration, and Proliferation in Primary Mouse Embryonic Fibroblasts

2006 ◽  
Vol 17 (11) ◽  
pp. 4675-4685 ◽  
Author(s):  
Linda Yang ◽  
Lei Wang ◽  
Yi Zheng
Keyword(s):  
Gene Targeting ◽  
Cell Lines ◽  
Cell Cycle Progression ◽  
Cell Model ◽  
Es Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Membrane Ruffling ◽  
Primary Mouse

Recent studies in Cdc42 knockout mouse embryonic stem (ES) cells and ES-derived fibroblastoid cell lines raise concern on a body of literature derived by dominant mutant expression approach in a variety of cell lines implicating mammalian Cdc42 as a key regulator of filopodia induction, directional migration and cell cycle progression. To resolve the physiological function of mammalian Cdc42, we have characterized the Cdc42−/− and Cdc42GAP−/− primary mouse embryonic fibroblasts (MEFs) produced by gene targeting as the Cdc42 loss- or gain-of-activity cell model. The Cdc42−/− cells were defective in filopodia formation stimulated by bradykinin and in dorsal membrane ruffling stimulated by PDGF, whereas the Cdc42GAP−/− cells displayed spontaneous filopodia. The Cdc42 loss- or gain-of-activity cells were defective in adhesion to fibronectin, wound-healing, polarity establishment, and migration toward a serum gradient. These defects were associated with deficiencies of PAK1, GSK3β, myosin light chain, and FAK phosphorylation. Furthermore, Cdc42−/− cells were defective in G1/S-phase transition and survival, correlating with deficient NF-κB transcription and defective JNK, p70 S6K, and ERK1/2 activation. These results demonstrate a different requirement of Cdc42 activity in primary MEFs from ES or ES-derived clonal fibroblastoid cells and suggest that Cdc42 plays cell-type–specific signaling roles.

scholarly journals Alkbh2 protects against lethality and mutation in primary mouse embryonic fibroblasts

DNA Repair ◽  
2012 ◽  
Vol 11 (5) ◽  
pp. 502-510 ◽  
Author(s):  
Stephanie L. Nay ◽  
Dong-Hyun Lee ◽  
Steven E. Bates ◽  
Timothy R. O’Connor
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Primary Mouse

scholarly journals Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts

Virology ◽  
2013 ◽  
Vol 442 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Lisa M. Mattei ◽  
Susan F. Cotmore ◽  
Peter Tattersall ◽  
Akiko Iwasaki
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Viral Control ◽  
Embryonic Fibroblasts ◽  
Primary Mouse

scholarly journals Nonviral Direct Conversion of Primary Mouse Embryonic Fibroblasts to Neuronal Cells

2012 ◽  
Vol 1 ◽  
pp. e32 ◽  
Author(s):  
Andrew F Adler ◽  
Christopher L Grigsby ◽  
Karina Kulangara ◽  
Hong Wang ◽  
Ryohei Yasuda ◽  
...  
Keyword(s):  
Neuronal Cells ◽  
Direct Conversion ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Primary Mouse

scholarly journals Loss of c-abl facilitates anchorage-independent growth of p53- and RB- deficient primary mouse embryonic fibroblasts

Oncogene ◽  
2004 ◽  
Vol 23 (52) ◽  
pp. 8527-8534 ◽  
Author(s):  
Jun Suzuki ◽  
Taiko Sukezane ◽  
Tsuyoshi Akagi ◽  
Maria Magdalena Georgescu ◽  
Mayumi Ohtani ◽  
...  
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Anchorage Independent Growth ◽  
Primary Mouse

scholarly journals LMP1 of Epstein-Barr Virus Induces Proliferation of Primary Mouse Embryonic Fibroblasts and Cooperatively Transforms the Cells with a p16-Insensitive CDK4 Oncogene

2000 ◽  
Vol 74 (2) ◽  
pp. 883-891 ◽  
Author(s):  
Xinhai Yang ◽  
Jonathan S. T. Sham ◽  
M. H. Ng ◽  
Sai-Wah Tsao ◽  
Dekai Zhang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Epstein Barr Virus ◽  
Growth Regulation ◽  
Target Cells ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Barr Virus ◽  
Primary Mouse ◽  
Lmp1 Gene ◽  
Epstein Barr

ABSTRACT The latent membrane protein LMP1 of Epstein-Barr virus (EBV) is often present in EBV-associated malignancies including nasopharyngeal carcinoma and Hodgkin's lymphoma. Previous work demonstrates that the LMP1 gene of EBV is sufficient to transform certain established rodent fibroblast cell lines and to induce the tumorigenicity of some human epithelial cell lines. In addition, LMP1 plays pleiotropic roles in cell growth arrest, differentiation, and apoptosis, depending on the background of the target cells. To examine the roles of LMP1 in cell proliferation and growth regulation in primary culture cells, we constructed a recombinant retrovirus containing an LMP1 gene. With this retrovirus, LMP1 was shown to stimulate the proliferation of primary mouse embryonic fibroblasts (MEF cells). It has a mitogenic activity for MEF cells, as demonstrated by an immediate induction of cell doubling time. In addition, it significantly extends the passage number of MEF cells to more than 30 after retroviral infection, compared with less than 5 for uninfected MEF cells. Furthermore, LMP1 cooperates with a p16-insensitive CDK4 R24C oncogene in transforming MEF cells. Our results provide the first evidence of the abilities of the LMP1 gene, acting alone, to effectively induce the proliferation of primary MEF cells and of its cooperativity with another cellular oncogene in transforming primary cells.

Primary mouse embryonic fibroblasts: A model of mesenchymal cartilage formation

2004 ◽  
Vol 200 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Christopher J. Lengner ◽  
Christoph Lepper ◽  
Andre J. van Wijnen ◽  
Janet L. Stein ◽  
Gary S. Stein ◽  
...  
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Cartilage Formation ◽  
Primary Mouse

Skp2 promotes adipocyte differentiation via a p27Kip1-independent mechanism in primary mouse embryonic fibroblasts

2009 ◽  
Vol 379 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Mitsuru Okada ◽  
Tamon Sakai ◽  
Takehiro Nakamura ◽  
Mimi Tamamori-Adachi ◽  
Shigetaka Kitajima ◽  
...  
Keyword(s):  
Adipocyte Differentiation ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Primary Mouse ◽  
Independent Mechanism
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