scholarly journals Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

10.3791/53850 ◽  
2016 ◽  
Author(s):  
André O. Hudson ◽  
Taylor C.M. Harkness ◽  
Michael A. Savka
Keyword(s):  
Functional Complementation ◽  
Complementation Analysis ◽  
Laboratory Exercise ◽  
Biochemical Pathways

scholarly journals Functional complementation analysis of yeast bc1 mutants . A study of the mitochondrial import of heterologous and hybrid proteins

1999 ◽  
Vol 264 (3) ◽  
pp. 825-832 ◽  
Author(s):  
Sandra van Wilpe ◽  
Hans Boumans ◽  
Gisele Lobo-Hajdu ◽  
Leslie A. Grivell ◽  
Jan A. Berden
Keyword(s):  
Functional Complementation ◽  
Complementation Analysis ◽  
Mitochondrial Import ◽  
Hybrid Proteins

scholarly journals Functional complementation analysis in potato via biolistic transformation with BAC large DNA fragments

2004 ◽  
Vol 13 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Maria Raffaella Ercolano ◽  
Agim Ballvora ◽  
Jürgen Paal ◽  
Hans-Henning Steinbiss ◽  
Francesco Salamini ◽  
...  
Keyword(s):  
Functional Complementation ◽  
Complementation Analysis ◽  
Biolistic Transformation ◽  
Dna Fragments

Laboratory Exercise on the Segregation of Flower Color and Related Genes Using Salpiglossis sinuata

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 555b-555
Author(s):  
Chiwon W. Lee
Keyword(s):  
Phenotypic Expression ◽  
Pollen Grains ◽  
Flower Color ◽  
Segregation Ratio ◽  
Crop Breeding ◽  
Flower Opening ◽  
Pigmentation Pattern ◽  
Laboratory Exercise ◽  
Demonstration Plant ◽  
Corolla Color

Velvet flower (Salpiglossis sinuata, Solanaceae) can be used as an excellent demonstration plant for horticultural crop breeding classes. Salpiglossis produces large trumpet-like flowers exhibiting an assortment of corolla color and pigmentation pattern. The pistil is large (3 to 4 cm long) with a sticky stigmatal tip and anthers can be easily emasculated prior to anthesis. The large pollen grains are shed in tetrads, which can be separated and individually placed on the stigma. It takes 8 to 9 weeks from seeding to blooming, with a prolific flowering cycle repeated in flushes. Numerous seeds (about 750/capsule) are obtained in 3 weeks after self- or cross-pollination. The influences of three genes that control flower color and pigmentation pattern can be conveniently demonstrated with their dominant and recessive alleles. The R gene controls flower color with red (RR or Rr) being dominant over yellow (rr) flower color. The D gene controls the density of pigmentation with solid (DD or Dd) color being dominant over dilute (dd) color. Corolla color striping is controlled by the St gene with striped (stst) being recessive to non-striped (StSt or Stst) pattern. For example, by using diploid lines of genotypes RRDD (red, solid), RRdd (red, dilute), or rrdd (yellow, dilute) and their crosses, students can easily learn a dominant phenotypic expression in the F1 hybrid and the digenic 9:3:3:1 segregation ratio in the F2 progeny. Another gene (C) that controls flower opening can also be used to show its influence on cleistogamous (closed, self-pollinated, CC or Cc) versus normal chasmogamous (open-pollinated, cc) corolla development. In addition, the induction and use of polyploid (4X, 3X) plants in plant breeding can be effectively demonstrated using this species.

A laboratory exercise using the microcomputer to determine nutrient recommendations and least-cost fertilizer blends

1984 ◽  
Vol 13 (1) ◽  
pp. 50-53
Author(s):  
Kenneth M. Oates ◽  
James Camberato ◽  
M. J. Vepraskas
Keyword(s):  
Laboratory Exercise

A Simple Botanical Key to Several Forage and Weedy Grass Seedlings For Use in a Student Laboratory Exercise

1972 ◽  
Vol 1 (1) ◽  
pp. 18-21
Author(s):  
J. R. George ◽  
G. E. Pepper
Keyword(s):  
Laboratory Exercise ◽  
Student Laboratory

Biochemical Pathways and Myometrial Cell Differentiation Leading to Nodule Formation Containing Collagen and Fibronectin

2016 ◽  
Vol 18 (2) ◽  
pp. 155-166 ◽  
Author(s):  
Liping Feng ◽  
Friederike Jayes ◽  
Lauren Johnson ◽  
David Schomberg ◽  
Phyllis Leppert
Keyword(s):  
Cell Differentiation ◽  
Nodule Formation ◽  
Myometrial Cell ◽  
Biochemical Pathways

Parameter Estimation By Using An Improved Bee Memory Differential Evolution Algorithm (Ibmde) To Simulate Biochemical Pathways

2013 ◽  
Vol 8 (999) ◽  
pp. 1-6
Author(s):  
Chuii Khim Chong ◽  
Mohd Saberi Mohamad ◽  
Safaai Deris ◽  
Mohd Shahir Shamsir ◽  
Lian En Chai ◽  
...  
Keyword(s):  
Parameter Estimation ◽  
Differential Evolution ◽  
Differential Evolution Algorithm ◽  
Biochemical Pathways ◽  
Evolution Algorithm

scholarly journals ‘Whole Organism’, Systems Biology, and Top-Down Criteria for Evaluating Scenarios for the Origin of Life

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 690
Author(s):  
Clifford F. Brunk ◽  
Charles R. Marshall
Keyword(s):  
Origin Of Life ◽  
Top Down ◽  
Chemical Systems ◽  
Biochemical Pathways ◽  
The Origin Of Life ◽  
Energy And Material Flows ◽  
Complex Scenario ◽  
Life On Earth ◽  
Physical Laws ◽  
Equilibrium Chemical

While most advances in the study of the origin of life on Earth (OoLoE) are piecemeal, tested against the laws of chemistry and physics, ultimately the goal is to develop an overall scenario for life’s origin(s). However, the dimensionality of non-equilibrium chemical systems, from the range of possible boundary conditions and chemical interactions, renders the application of chemical and physical laws difficult. Here we outline a set of simple criteria for evaluating OoLoE scenarios. These include the need for containment, steady energy and material flows, and structured spatial heterogeneity from the outset. The Principle of Continuity, the fact that all life today was derived from first life, suggests favoring scenarios with fewer non-analog (not seen in life today) to analog (seen in life today) transitions in the inferred first biochemical pathways. Top-down data also indicate that a complex metabolism predated ribozymes and enzymes, and that full cellular autonomy and motility occurred post-LUCA. Using these criteria, we find the alkaline hydrothermal vent microchamber complex scenario with a late evolving exploitation of the natural occurring pH (or Na+ gradient) by ATP synthase the most compelling. However, there are as yet so many unknowns, we also advocate for the continued development of as many plausible scenarios as possible.

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