scholarly journals Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

10.3791/57006 ◽  
2018 ◽  
Author(s):  
George M. Solomon ◽  
Inez Bronsveld ◽  
Kathryn Hayes ◽  
Michael Wilschanski ◽  
Paola Melotti ◽  
...  
Keyword(s):  
Potential Difference ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference ◽  
Standardized Measurement

Nitric oxide-mediated regulation of transepithelial sodium and chloride transport in murine nasal epithelium

1999 ◽  
Vol 276 (3) ◽  
pp. L466-L473 ◽  
Author(s):  
Heather L. Elmer ◽  
Kristine G. Brady ◽  
Mitchell L. Drumm ◽  
Thomas J. Kelley
Keyword(s):  
Nitric Oxide ◽  
Chloride Transport ◽  
Chloride Secretion ◽  
Inos Expression ◽  
Potential Difference ◽  
Nasal Epithelium ◽  
Sodium Absorption ◽  
No Production ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

Transepithelial ion transport is regulated by a variety of cellular factors. In light of recent evidence that nitric oxide (NO) production is decreased in cystic fibrosis airways, we examined the role of NO in regulating sodium and chloride transport in murine nasal epithelium. Acute intervention with the inducible NO synthase (iNOS)-selective inhibitor S-methylisothiourea resulted in an increase of amiloride-sensitive sodium absorption observed as a hyperpolarization of nasal transepithelial potential difference. Inhibition of iNOS expression with dexamethasone also hyperpolarized transepithelial potential difference, but only a portion of this increase proved to be amiloride sensitive. Chloride secretion was significantly inhibited in C57BL/6J mice by the addition of both S-methylisothiourea and dexamethasone. Mice lacking iNOS expression [NOS2(−/−)] also had a decreased chloride-secretory response compared with control mice. These data suggest that constitutive NO production likely plays some role in the downregulation of sodium absorption and leads to an increase in transepithelial chloride secretion.

Calcium secretion in canine tracheal mucosa

1985 ◽  
Vol 59 (4) ◽  
pp. 1191-1195 ◽  
Author(s):  
F. J. Al-Bazzaz ◽  
T. Jayaram
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Open Circuit ◽  
Potential Difference ◽  
Secretory Process ◽  
Metabolic Inhibitor ◽  
Tracheal Mucosa ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

Calcium (Ca) affects many cellular functions of the respiratory tract mucosa and might alter the viscoelastic properties of mucus. To evaluate Ca homeostasis in a respiratory epithelium we investigated transport of Ca by the canine tracheal mucosa. Mucosal tissues were mounted in Ussing-type chambers and bathed with Krebs-Henseleit solution at 37 degrees C. Unidirectional fluxes of 45Ca were determined in tissues that were matched by conductance and short-circuit current (SCC). Under short-circuit conditions there was a significant net Ca secretion of 1.82 +/- 0.36 neq . cm-2 . h-1 (mean +/- SE). Under open-circuit conditions, where the spontaneous transepithelial potential difference could attract Ca toward the lumen, net Ca secretion increased significantly to 4.40 +/- 1.14 compared with 1.54 +/- 1.17 neq . cm-2 . h-1 when the preparation was short-circuited. Addition of a metabolic inhibitor, 2,4-dinitrophenol (2 mM in the mucosal bath), decreased tissue conductance and SCC and slightly decreased the unidirectional movement of Ca from submucosa to lumen. Submucosal epinephrine (10 microM) significantly enhanced Ca secretion by 2.0 +/- 0.63 neq . cm-2 . h-1. Submucosal ouabain (0.1 mM) failed to inhibit Ca secretion. The data suggest that canine tracheal mucosa secretes Ca; this secretory process is augmented by epinephrine or by the presence of a transepithelial potential difference as found under in vivo conditions.

scholarly journals A new device for in vivo measurement of nasal transepithelial potential difference in cystic fibrosis patients and normal subjects

1997 ◽  
Vol 10 (7) ◽  
pp. 1631-1636 ◽  
Author(s):  
O. Duperrex ◽  
P-Y. Berclaz ◽  
D. Bertrand ◽  
J.S. Lacroix ◽  
N. Pochon ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Normal Subjects ◽  
Potential Difference ◽  
New Device ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

scholarly journals Frequency Spectrum of Transepithelial Potential Difference Reveals Transport-Related Oscillations

2009 ◽  
Vol 97 (6) ◽  
pp. 1530-1537 ◽  
Author(s):  
Nicolás Montalbetti ◽  
Jorge Fischbarg
Keyword(s):  
Frequency Spectrum ◽  
Potential Difference ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

scholarly journals Parallel effects of VX-770 on transepithelial potential difference in vitro and in vivo

2010 ◽  
Vol 9 ◽  
pp. S20 ◽  
Author(s):  
S.M. Rowe ◽  
J.P. Clancy ◽  
M. Boyle ◽  
F. Van Goor ◽  
C. Ordonez ◽  
...  
Keyword(s):  
Potential Difference ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

Factors influencing transepithelial potential difference in mammalian distal tubule

1978 ◽  
Vol 234 (3) ◽  
pp. F182-F191 ◽  
Author(s):  
J. P. Hayslett ◽  
E. L. Boulpaep ◽  
G. H. Giebisch
Keyword(s):  
Electrical Properties ◽  
Cell Membrane ◽  
Distal Tubule ◽  
Potential Difference ◽  
Ion Concentration ◽  
Luminal Cell ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference ◽  
Factors Influencing ◽  
Average Slope

Studies were performed to examine some electrical properties of the mammalian distal tubule. Experiments were performed to evaluate the influence of changes in ion concentration in luminal fluid on transepithelial potential difference. Variations in the concentration of Na+ and K+, near the physiologic range, resulted in only modest changes in potential difference. During changes in Na+ the average slope, deltaV/ln C2/C1, was -1.52 +/- 0.39 mV and during variations in K+ the slope was -5.60 +/- 0.95 mV. Changes in Cl- concentration had no effect on transepithelial potential difference. Since the sum of transferase numbers did not equal 1, it seems likely that potential difference is influenced by a shunt current due to cellular rheogenic pumps. These data, therefore, indicate that variations in ion concentration in tubular fluid of the mammalian distal tubule do not significantly influence the resting transepithelial potential difference, and they suggest that analysis of permselective properties of the luminal cell membrane by transepithelial electrophysiologic approaches is probably invalid.

The influence of acetylsalicylic acid on the transepithelial potential difference of gastric mucosa in children

1985 ◽  
Vol 63 (4) ◽  
pp. 184-187
Author(s):  
N. Nikolaides ◽  
A. König ◽  
E. H. Ballke ◽  
B. Griefahn ◽  
K. Jährig
Keyword(s):  
Gastric Mucosa ◽  
Acetylsalicylic Acid ◽  
Potential Difference ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

The transepithelial potential difference in intact larval and adult salamanders

1970 ◽  
Vol 33 (1) ◽  
pp. 209-212 ◽  
Author(s):  
Ronald H Alvarado ◽  
Daniel F Stiffler
Keyword(s):  
Potential Difference ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference
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