scholarly journals Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

10.3791/58109 ◽  
2018 ◽  
Author(s):  
Nikolai Klena ◽  
Davide Gambarotto ◽  
Maeva Le Guennec ◽  
Susanne Borgers ◽  
Paul Guichard ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Particle ◽  
Single Particle Reconstruction ◽  
Particle Reconstruction

scholarly journals SubspaceEM: A fast maximum-a-posteriori algorithm for cryo-EM single particle reconstruction

2015 ◽  
Vol 190 (2) ◽  
pp. 200-214 ◽  
Author(s):  
Nicha C. Dvornek ◽  
Fred J. Sigworth ◽  
Hemant D. Tagare
Keyword(s):  
Single Particle ◽  
Maximum A Posteriori ◽  
A Posteriori ◽  
Single Particle Reconstruction ◽  
Particle Reconstruction

The Laplacian of Gaussian and arbitrary z-crossings approach applied to automated single particle reconstruction

2007 ◽  
Vol 159 (1) ◽  
pp. 122-134 ◽  
Author(s):  
D WOOLFORD ◽  
B HANKAMER ◽  
G ERICKSSON
Keyword(s):  
Single Particle ◽  
Single Particle Reconstruction ◽  
Particle Reconstruction

scholarly journals 3-D Structure of Cellulose Microfibril in Wood Using Electron Tomography and Single Particle Reconstruction Methods

2013 ◽  
Vol 19 (S2) ◽  
pp. 72-73
Author(s):  
M. Reza ◽  
P. Engelhardt ◽  
J. Ruokolainen
Keyword(s):  
Single Particle ◽  
Cellulose Microfibril ◽  
Electron Tomography ◽  
Single Particle Reconstruction ◽  
Reconstruction Methods ◽  
Particle Reconstruction

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Single Particle Reconstruction of the Vault from TEP1 Knockout Mice and Refinement of the Computationally Selected Vault Barrel

2000 ◽  
Vol 6 (S2) ◽  
pp. 268-269
Author(s):  
P.L. Stewart ◽  
L.B. Kong ◽  
A.C. Siva ◽  
V.A. Kickhoefer ◽  
L. Harrington ◽  
...  
Keyword(s):  
Single Particle ◽  
Negative Influence ◽  
Cancer Drug ◽  
Molecular Architecture ◽  
Single Particle Reconstruction ◽  
Macromolecular Transport ◽  
Reconstruction Methods ◽  
Anti Cancer ◽  
Difference Imaging ◽  
Particle Reconstruction

The vault complex is a ubiquitous and predominantly cytoplasmic 13 MDa ribonucleoprotein assembly, composed of three proteins (TEP1, 240 kDa; VPARP, 193 kDa; and MVP, 100 kDa) and an untranslated RNA (vRNA) Although the basic cellular function of the vault is still unclear, recently it has been shown that induction of the major vault protein (MVP) has a direct negative influence on the nuclear uptake of the anti-cancer drug, doxorubicin. We have been applying cryo-EM single particle reconstruction methods to study the structure and molecular architecture of this cellular component. A published reconstruction of the intact rat vault at 31 Å resolution revealed that the complex is hollow and is structurally well suited to serve in macromolecular transport or sequestration. Higher resolution, 23 Å, was achieved for a reconstruction of the RNase-treated rat vault and difference imaging with the intact rat vault localized the vRNA to the ends of the vault caps.

scholarly journals SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs

2008 ◽  
Vol 3 (12) ◽  
pp. 1941-1974 ◽  
Author(s):  
Tanvir R Shaikh ◽  
Haixiao Gao ◽  
William T Baxter ◽  
Francisco J Asturias ◽  
Nicolas Boisset ◽  
...  
Keyword(s):  
Image Processing ◽  
Electron Micrographs ◽  
Single Particle ◽  
Biological Macromolecules ◽  
Single Particle Reconstruction ◽  
Particle Reconstruction

scholarly journals Extending the Limits of Single Particle Reconstruction

2009 ◽  
Vol 15 (S2) ◽  
pp. 566-567
Author(s):  
S Ludtke ◽  
M Schmid ◽  
W Chiu
Keyword(s):  
Single Particle ◽  
Single Particle Reconstruction ◽  
Particle Reconstruction

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals Development of Nanostructured Electron Microscopy Grids for Time Resolved Single Particle Reconstruction for Transmission Electron Microscopy

2006 ◽  
Vol 12 (S02) ◽  
pp. 1130-1131
Author(s):  
D Barnard ◽  
TR Shaikh ◽  
K Buttle ◽  
V Pushparaj ◽  
D Ye ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Single Particle ◽  
Time Resolved ◽  
Single Particle Reconstruction ◽  
Transmission Electron ◽  
Particle Reconstruction

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

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