scholarly journals Identification of the First Nuclear Male Sterility Gene ( Male‐sterile 9 ) in Sorghum

2019 ◽  
Vol 12 (3) ◽  
pp. 190020 ◽  
Author(s):  
Junping Chen ◽  
Yinping Jiao ◽  
Haydee Laza ◽  
Paxton Payton ◽  
Doreen Ware ◽  
...  
Keyword(s):  
Male Sterility ◽  
Male Sterile ◽  
Nuclear Male Sterility ◽  
Sterility Gene ◽  
Male Sterility Gene

scholarly journals Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis

2020 ◽  
Vol 10 (4) ◽  
pp. 1309-1318
Author(s):  
Tzu-Kai Lin ◽  
Ya-Ping Lin ◽  
Shun-Fu Lin
Keyword(s):  
Male Sterility ◽  
Fine Mapping ◽  
Genotyping By Sequencing ◽  
Snp Markers ◽  
Hybrid Seed Production ◽  
Spontaneous Mutant ◽  
Male Sterile ◽  
Sterility Gene ◽  
Intron 4 ◽  
Male Sterility Gene

Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMsa, BrMsb, and BrMsc. The male sterility locus has been mapped to chromosome A07 in BC1 and F2 populations through genotyping by sequencing. Fine mapping in a total of 1,590 F2 plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F2 population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait.

Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea

Genome ◽  
2005 ◽  
Vol 48 (5) ◽  
pp. 848-854 ◽  
Author(s):  
Xiaowu Wang ◽  
Ping Lou ◽  
Guusje Bonnema ◽  
Baojun Yang ◽  
Hangjun He ◽  
...  
Keyword(s):  
Male Sterility ◽  
Brassica Oleracea ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Dominant Male ◽  
Male Sterile ◽  
Sterility Gene ◽  
Male Sterility Gene

The dominant male sterility gene Ms-cd1 (c, cabbage; d, dominant) was identified as a spontaneous mutation in the spring cabbage line 79-399-3. The Ms-cd1 gene is successfully applied in hybrid seed production of several Brassica oleracea cultivars in China. Amplified fragment length polymorphism (AFLP) technology was used to identify markers linked to the Ms-cd1 gene in bulks of male-sterile and male-fertile individuals of a segregating BC3 population and in a near-isogenic population of 25 male-sterile plants. Twelve markers within a 20-cM interval proximal to the Ms-cd1 gene were identified, 5 of which can be used to select homozygous male-sterile Ms-cd1/ Ms-cd1 plants. Three AFLP markers and 3 sequence characterized amplified region markers that were linked to MS-cd1 mapped onto linkage group O9, corresponding to chromosome 3 of B. oleracea. This region corresponds to the top of chromosome 5 in Arabidopsis thaliana.Key words: Brassica oleracea, dominant male-sterility gene, bulk segregant analysis, amplified fragment length polymorphism (AFLP), genetic mapping.

scholarly journals Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population

2013 ◽  
Vol 13 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Darika Bunphan ◽  
Prasit Jaisil ◽  
Jirawat Sanitchon
Keyword(s):  
Male Sterility ◽  
Sweet Sorghum ◽  
Segregation Ratio ◽  
Male Sterile ◽  
Mendelian Segregation ◽  
Breeding Programs ◽  
Sterility Gene ◽  
The One ◽  
B Lines ◽  
Male Sterility Gene

The objectives of this study were to verify the use of LW7 marker in identifying maintainer lines (B-lines) and restorer lines (R-lines) in grain sorghum and sweet sorghum, and to identify B-lines in the F2, BC1F2 and BC2F2 generations. Twenty five accessions of sorghum were evaluated, and LW7 marker correctly identified accessions which presented male sterility gene (rf4) in Suphan Buri1 and 03B cultivars; moreover, these genotypes did not show 779 bp band. The cross between Suphan Buri1 and a male-sterile line (A-line) 03A resulted in a sterilized male, confirming the usefulness of the marker in breeding programs. B-lines in the F2, BC1F2 and BC2F2 generations were identified by LW7 marker. The segregation ratio of 3:1 for male fertility and male sterility in the progenies of the three generations supported the one-gene model of Mendelian segregation. The use of marker assisted selection was successful for line development of sweet sorghum with male sterility.

scholarly journals FUNCTIONAL MALE STERILITY IN EGGPLANT: INHERITANCE AND LINKAGE TO THE PURPLE FRUIT COLOR GENE

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1168f-1168
Author(s):  
Sharad C. Phatak ◽  
Jinsheng Liu ◽  
Casimir A. Jaworski ◽  
A. Fazal Sultanbawa
Keyword(s):  
Male Sterility ◽  
Fruit Color ◽  
Male Sterile ◽  
Fruit Peel ◽  
Violet Color ◽  
Sterility Gene ◽  
Functional Male Sterility ◽  
Purple Color ◽  
Male Sterility Gene ◽  
Color Gene

The functional male sterile (fms) eggplant (Solanum melohgena L.) germplasm UGA 1-MS was crossed with two cultivars, `UGA 18 White' and `Florida Market' with normal anthers to derive F1, F2, and BC populations. Functional male sterility (fms) was governed by a single recessive allele. The gene symbol fms is proposed for this male sterile characteristic. The functional male sterility gene was linked to purple fruit color at the X/x locus. Our observations also revealed that the purple or violet color ware not only on the fruit peel, but also on the anthers and leaf buds if the eggplant fruit was purple or violet. In the transmission of parents and progenies of the cross of UGA 1-MS × `UGA 18 White', the purple line on the anther and leaf bud purple color ware tightly associated with fruit purple color. Thus, it is assumed that the allele X controls not only purple fruit, but also the expression of the purple line on the anther and purple leaf bud.

Molecular mapping of nuclear male-sterility gene ms-1 in muskmelon (Cucumis melo L.)

2019 ◽  
Vol 95 (2) ◽  
pp. 162-168 ◽  
Author(s):  
Manpreet Singh ◽  
Sat Pal Sharma ◽  
Navraj Kaur Sarao ◽  
Simranjot Kaur ◽  
Parveen Chhuneja
Keyword(s):  
Male Sterility ◽  
Cucumis Melo ◽  
Molecular Mapping ◽  
Nuclear Male Sterility ◽  
Sterility Gene ◽  
Cucumis Melo L ◽  
Male Sterility Gene
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