scholarly journals Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania

2014 ◽  
Vol 8 (06) ◽  
pp. 694-698 ◽  
Author(s):  
Alexandru Rafila ◽  
Alexander Indra ◽  
Gabriel Adrian Popescu ◽  
Günther Wewalka ◽  
Franz Allerberger ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Hospital Mortality ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Binary Toxin ◽  
Notifiable Disease ◽  
Repeat Analysis ◽  
Ribotype 027 ◽  
Toxigenic Strains ◽  
Pcr Ribotype 027

Introduction: Little is known about prevailing ribotypes of Clostridium difficile infection in Romania where CDI is not a mandatory notifiable disease. Methodology: We studied 64 non-duplicate C. difficile isolates from patients hospitalised at the National Institute of Infectious Diseases, Bucharest, Romania between March 2011 and March 2012. Results: Sixty-three of the 64 C. difficile isolates produced toxins A and B whereas 44 (69%) isolates produced a binary toxin. Ribotype 027 accounted for 43 (68%) of the 63 toxigenic strains. The remaining 20 isolates belonged to ribotypes 018 (n = 9), 012 (n = 3), and, with one isolate each, 014, 031, 081, 416, 433, 500, 507 and PR03035 (new ribotype). Information on hospital mortality was available for 62 of the 64 patients; among these 62 cases, 4 (6.4%) ended fatal. Recurrence was documented for 11 (18.3%) of the 60 patients for whom this information was available. Multilocus variable-number tandem repeat analysis of the 43 isolates of ribotype 027 yielded a unique cluster for the Romanian isolates when compared to Austrian or Italian isolates. Conclusion: Our findings sustain the hypothesis of a recent emerged outbreak of C. difficile PCR ribotype 027 infections in the area of Bucharest.

scholarly journals Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals

2006 ◽  
Vol 44 (7) ◽  
pp. 2558-2566 ◽  
Author(s):  
J. W. Marsh ◽  
M. M. O'Leary ◽  
K. A. Shutt ◽  
A. W. Pasculle ◽  
S. Johnson ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Repeat Analysis

scholarly journals Typing and Subtyping of Clostridium difficile Isolates by Using Multiple-Locus Variable-Number Tandem-Repeat Analysis

2006 ◽  
Vol 45 (3) ◽  
pp. 1024-1028 ◽  
Author(s):  
R. J. van den Berg ◽  
I. Schaap ◽  
K. E. Templeton ◽  
C. H. W. Klaassen ◽  
E. J. Kuijper
Keyword(s):  
Clostridium Difficile ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Two cases of fulminant colitis due to binary toxin-positive Clostridium difficile that are not PCR ribotype 027 or type 078

2013 ◽  
Vol 62 (9) ◽  
pp. 1486-1489 ◽  
Author(s):  
Yasuaki Tagashira ◽  
Haru Kato ◽  
Mitsutoshi Senoh ◽  
Akira Nakamura
Keyword(s):  
Clostridium Difficile ◽  
Binary Toxin ◽  
Fulminant Colitis ◽  
Toxin B ◽  
Ribotype 027 ◽  
Base Pair Deletion ◽  
Pcr Ribotyping ◽  
Single Base Pair ◽  
Ribotype 078 ◽  
Pcr Ribotype 027

Two cases of fulminant colitis due to Clostridium difficile occurred within ten weeks of each other on the same ward of a hospital in Japan. The patients died 2 and 4 days after the onset of colitis. C. difficile isolates obtained from both patients were toxin A-positive, toxin B-positive and binary toxin-positive. These isolates yielded identical results by both PCR ribotyping and slpA sequence typing. However, the banding patterns and slpA sequences of the isolates differed from those of PCR ribotype 027, as well as those of PCR ribotype 078. The tcdC sequences of the isolate differed from those of C. difficile 027, but a single base-pair deletion at position 117 and an 18 bp deletion, both of which were identical to the sequence of the reference strain of 027, were found. This type may be a new hypervirulent strain, but further studies of the epidemiology and pathogenicity of the strain are needed.

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