Bioethanol Production from Hydrodictyon reticulatum by Fed-Batch Fermentation Using Saccharomyces cerevisiae KCTC7017

2017 ◽  
Vol 27 (6) ◽  
pp. 1112-1119 ◽  
Author(s):  
Seul Ki Kim ◽  
Cuong Mai Nguyen ◽  
Eun Hye Ko ◽  
In-Chul Kim ◽  
Jin-Seog Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Fermentation ◽  
Bioethanol Production ◽  
Fed Batch ◽  
Hydrodictyon Reticulatum ◽  
Fed Batch Fermentation

scholarly journals Efficient Alcoholic Conversion of Glycerol by Engineered Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Takashi Watanabe ◽  
Sadat M. R. Khattab
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nicotinamide Adenine Dinucleotide ◽  
Batch Fermentation ◽  
Plant Biomass ◽  
Glycerol Oxidation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Engineered Saccharomyces Cerevisiae ◽  
Biomass Decomposition ◽  
Engineered Strain

Glycerol is an eco-friendly solvent that enhances plant biomass decomposition via glycerolysis in many pretreatment methods. Nonetheless, the lack of efficient conversion of glycerol by natural Saccharomyces cerevisiae hinders its use in these methods. Here, we have aimed to develop a complete strategy for the generation of efficient glycerol-converting yeast by modifying the oxidation of cytosolic nicotinamide adenine dinucleotide (NADH) by an O2-dependent dynamic shuttle, while abolishing both glycerol phosphorylation and biosynthesis. By following a vigorous glycerol oxidation pathway, the engineered strain increased the conversion efficiency (CE) to up to 0.49 g ethanol/g glycerol (98% of theoretical CE), with production rate > 1 g×L×h, when glycerol was supplemented in a single fed-batch fermentation in a rich medium. Furthermore, the engineered strain fermented a mixture of glycerol and glucose, producing > 86 g/L bioethanol with 92.8% CE. To our knowledge, this is the highest ever reported titer in this field. Notably, this strategy changed conventional yeast from a non-grower on minimal medium containing glycerol to a fermenting strain with productivity of 0.25-0.5 g×L×h and 84-78% CE, which converted 90% of the substrate to products. Our findings may improve the utilization of glycerol in several eco-friendly biorefinery approaches.

Sugarcane must fed-batch fermentation by Saccharomyces cerevisiae: impact of sterilized and non-sterilized sugarcane must

2019 ◽  
Vol 112 (8) ◽  
pp. 1177-1187
Author(s):  
Maria Letícia Bonatelli ◽  
Jaciane Lutz Ienczak ◽  
Carlos Alberto Labate
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation
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