Relationship Between Age and Pleural Fluid Adenosine Deaminase Activity in Patients with Tuberculous Pleural Effusion

2002 ◽  
Vol 52 (6) ◽  
pp. 608 ◽  
Author(s):  
Chong Ju Kim ◽  
Kyu Min Yeon ◽  
Shin Tae Kim ◽  
Joon Ho Wang ◽  
Kwang Ha Yoo
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Tuberculous Pleural Effusion ◽  
Adenosine Deaminase Activity

scholarly journals Diagnostic significance of pleural fluid adenosine deaminase activity in tuberculous pleurisy

1970 ◽  
Vol 5 (1) ◽  
pp. 1-5
Author(s):  
Sharmeen Ahmed ◽  
Reaz Fatema ◽  
Ahmed Abu Saleh ◽  
Mumayun Sattar ◽  
Md Ruhul Amin Miah
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Colorimetric Method ◽  
Diagnostic Methods ◽  
Test Accuracy ◽  
Diagnostic Significance ◽  
Tuberculous Pleural Effusion ◽  
Adenosine Deaminase Activity ◽  
Significant Difference

Diagnosis of tuberculous pleural effusion (TPE) is difficult because of its non-specific clinical presentation and insufficient efficiency of conventional diagnostic methods. The study was carried out to evaluate the utility of adenosine deaminase (ADA) activity in pleural fluid for the diagnosis of TPE. ADA activity was measured in pleural fluid of 103 pleural effusion patients by colorimetric method using a commercial ADA assay kit. The diagnosis of TPE was made from pleural fluid examinations (including cytology, biochemistry, and bacteriology) and pleural biopsy. Patient with negative result of this methods were diagnosed by response of empirical treatment. Out of 130 cases, 62 (61.1%) had TPE and the remaining 41 (39.8%) had pleural effusion due to non tuberculous diseases. There was statistically significant difference (p < 0.001) between the mean of pleural fluid ADA levels (70.82±22.54 U/L) in TPE group and (30.07±22.93 U/L) in non-TPE group. Of 62 TPE cases, microscopy for AFB and culture for M.tuberculosis in pleural fluid revealed positivity in 9.6% and 22.5% cases respectively, and biopsy of pleura showed typical epithelioid granuloma in only 43.5% cases. The cut-off value of ADA for diagnosing TPE was 40 U/L using a ROC curve, with a sensitivity of 94% and specificity of 88%. Positive and negative predictive value of ADA assay were 92% and 90% respectively. The overall test accuracy was 90%. Pleural fluid ADA assay is therefore a simple, rapid, highly sensitive and specific adjunct test for diagnosis of TPE. DOI: http://dx.doi.org/10.3329/imcj.v5i1.9852   Ibrahim Med. Coll. J. 2011; 5(1): 1-5 Keywords: Pleural fluid; adenosine deaminase; tuberculous pleural effusion

scholarly journals Pleural Fluid Adenosine Deaminase Values in Diagnosis of Tuberculous Pleural Effusion in South Khorasan Province

2016 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sayyed Gholam Reza Mortazavi-Moghaddam ◽  
Gholam Reza Sharifzadeh ◽  
Mohammad Reza Rezvani
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Tuberculous Pleural Effusion ◽  
South Khorasan

scholarly journals CORRELATION BETWEEN ADENOSINE DEAMINASE ACTIVITY IN PLEURAL FLUID AND SERUM OF PATIENTS WITH PLEURAL EFFUSION

2018 ◽  
Vol 24 (2) ◽  
pp. 180
Author(s):  
Pande Putu Ayu Patria Dewi ◽  
Aryati Aryati ◽  
Leonita Anniwati ◽  
Isnin Anang Marhana
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Gastrointestinal Disease ◽  
Pleural Space ◽  
Systemic Diseases ◽  
Cross Sectional ◽  
Adenosine Deaminase Activity ◽  
Abnormal Accumulation ◽  
Cross Sectional Design

Pleural effusion is an abnormal accumulation of fluid in the pleural space resulting from increased production of fluid or decreased resorption of fluid in the pleural space. Pleural effusion can be caused by infectious diseases, malignancies, collagen disease, gastrointestinal disease, heart disease and other causes such as medication. Adenosine Deaminase (ADA) is an enzyme involved in the catabolism of purines. This enzyme can be measured in pleural fluid, serum and other body fluids such as cerebrospinal and ascites fluid. The aim of this study was to analyze the correlation between adenosine deaminase activity in pleural fluid and serum in patients with pleural effusion. This research was an observational study with a cross-sectional design. Examination of ADA activity was performed in pleural fluid and serum. Adenosine deaminase activity was examined using photometric methods (Non-Giusti), using Diazyme reagent by TMS 24i Premium. Subjects were 46 patients with pleural effusion caused by malignancies, tuberculosis and systemic diseases. Mean±SD ADA activity for all pleural effusion samples in serum was 13.037± 8.365 (0.5-34.1) U//L and pleural fluid 30.843± 28.860 U//L (1.3-140.8). No correlation between ADA activity in serum and pleural fluid (r=0.173, p= 0.252) was found in all samples. No correlation between ADA activity in serum and pleural fluis was found in malignancies (r=0.109, p=0.630), tuberculosis (r= 0.366, p=0.123), systemic diseases (r =0.466, p=0.429) and non-tuberculosis group (r=0.126, p=0.532). There was no correlation between pleural fluid ADA activity and serum. 

Diagnostic Accuracy of Tuberculous Pleural Effusion by Combining Adenosine Deaminase Assay and Lymphocyte/ Neutrophil Ratio in Pleural Fluid

2018 ◽  
Vol 7 (7) ◽  
pp. 831-834
Author(s):  
Kande Srinivasulu ◽  
◽  
Aruna Kumari Vadlavalli ◽  
Rajendra Prasad Jalagam ◽  
Nidudhavelu Gopichand ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Diagnostic Accuracy ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Tuberculous Pleural Effusion

scholarly journals Optimising the utility of pleural fluid adenosine deaminase for the diagnosis of tuberculous pleural effusion

2020 ◽  
pp. 201010582097899
Author(s):  
Nai-Chien Huan ◽  
Inn Shih Khor ◽  
Hema Yamini Ramarmuty ◽  
Ming Yao Lim ◽  
Kai Choon Ng ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Surrogate Marker ◽  
Queen Elizabeth Hospital ◽  
Tuberculous Pleural Effusion ◽  
Initial Work ◽  
The Mean ◽  
N 93 ◽  
Work Up

Introduction: Pleural fluid adenosine deaminase (pfADA) is a simple, rapid and inexpensive surrogate marker for tuberculous pleural effusion (TPE). A nationwide cut-off of 40 U/L is currently used based on overseas data. There is a need to optimise the diagnostic utility of pfADA by establishing a local cut-off value. In this study, we aimed to describe the demographics and clinical characteristics of patients with TPE and non-TPE; determine the sensitivity and specificity of current pfADA of 40 U/L; and establish a new local pfADA cut-off for TPE. Methods: We conducted a single-centre, observational, prospective study of patients with exudative pleural effusion and pfADA measured from 1 October 2019 to 30 April 2020 at Queen Elizabeth Hospital, Malaysia. Results: The diagnosis of analysed patients ( n = 93) included TPE ( n = 41), malignancy ( n = 28), parapneumonic effusion ( n = 12) and other causes ( n = 12). The mean pfADA was 51.15 U/L (standard deviation (SD) = 13.77) among TPE group and 18.86 U/L (SD = 12.33) among non-TPE. When analysis was restricted to TPE patients, the local pfADA cut-off is 29.6 U/L, with a sensitivity of 97.6% and specificity of 90.4%. The current pfADA of 40 U/L has a sensitivity of 87.8% and specificity of 92.3%. Conclusion: We established a local pfADA cut-off of 29.6 U/L for TPE. Optimising the utility of pfADA helps to enhance clinicians’ treatment confidence of TPE when initial work-up is inconclusive.

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