EFFECTS OF HERBICIDES OF DIFFERENT MODES OF ACTION ON LEAF-CELL MEMBRANE PERMEABILITY IN PHASEOLUS VULGARIS

1980 ◽  
Vol 60 (2) ◽  
pp. 613-620 ◽  
Author(s):  
JACINTA CROWLEY ◽  
G. N. PRENDEVILLE
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Leaf Damage ◽  
Cell Permeability ◽  
Cell Membrane Permeability ◽  
Leaf Cell ◽  
Visible Injury ◽  
Modes Of Action ◽  
2,4 Dichlorophenoxyacetic Acid

Leakage of electrolytes from leaf discs of treated Phaseolus vulgaris L. plants was used to study the effects of several herbicides of different modes of action on leaf-cell membrane permeability. Linuron (N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea), prometryne (4,6-bisisopropylamino-2-methylthio-1,3,5-triazine), bromacil (5,bromo-6-methyl-3-(1-methyl-n-propyl) uracil), sodium azide and dalapon (2,2-dichloropropionic acid) increased leaf-cell permeability at 24 h after treatment and this occurred without appearance of leaf necrosis. Glyphosate (N-(phosphonomethyl) glycine) increased leaf-cell permeability at 96 h and this was always associated with visible injury, including wilting. Paraquat (1,1-dimethyl-4,4-bipyridylium) at 10−5M increased leaf-cell permeability 48 h after treatment, without apparent leaf damage, but at higher concentrations, increased permeability was always associated with visible effects. Chlorpropham (isopropyl N-(d)3-chlorophenyl) carbamate), picloram (4-amino-3,5,6-trichloropicolinic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid) did not alter cell permeability even though epinastic symptoms in leaves became evident 24 h after treatment with picloram and 2,4-D.

EFFECT OF DICHLOFOP METHYL ON LEAF-CELL MEMBRANE PERMEABILITY IN WILD OAT, BARLEY AND WHEAT

1979 ◽  
Vol 59 (1) ◽  
pp. 275-277 ◽  
Author(s):  
JACINTA CROWLEY ◽  
G. N. PRENDEVILLE
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Foliar Application ◽  
Cell Membrane Permeability ◽  
Wild Oat ◽  
Leaf Cell ◽  
Visible Injury ◽  
Leaf Discs ◽  
Wild Oats

Leakage of electrolytes from leaf discs of treated wild oats, barley and wheat plants was the criterion used to compare the effect of dichlofop methyl 2-{4-(2, 4-dichlorophenoxy) phenoxy} propionate on leaf-cell membrane permeability in these species. Leakage of electrolytes from wild oats and barley occurred within 12 h and increased up to 96 h following foliar application of the chemical at the rate of 0.112 kg/ha and 0.56 kg/ha, while in wheat it occurred only at the highest rate and after an interval of 48 h. Increases in leaf-cell membrane permeability occurred in each of the three species before the appearance of visible injury such as discoloration or necrotic areas in leaf tips.

EXTH-74. INCREASING CANCER CELL MEMBRANE PERMEABILITY THROUGH APPLICATION OF TUMOR TREATING FIELDS (TTFIELDS)

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi180-vi180
Author(s):  
Tali Voloshin ◽  
Bella Koltun ◽  
Lilach Koren ◽  
Yaara Porat ◽  
Alexandra Volodin ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Permeability ◽  
Cell Membrane Permeability ◽  
Intracellular Accumulation ◽  
Tumor Treating Fields ◽  
Cell Counts ◽  
Resistant Cells

Abstract INTRODUCTION Tumor Treating Fields (TTFields) are intermediate frequency, alternating electric fields with anti-mitotic effects on cancerous cells. TTFields are delivered non-invasively through arrays placed on the skin at the tumor region. TTFields therapy is approved in several territories for treatment of glioblastoma (GBM) and mesothelioma. Recently, TTFields have been shown to increase GBM cell membrane permeability. The current study aimed to explore this effect in multiple cell lines and examine the potential benefits of combining TTFields with other anticancer agents. METHODS TTFields were delivered to GBM (U-87 MG), uterine sarcoma (MES-SA), and breast adenocarcinoma (MCF-7) cell lines for 72hr across a range of frequencies (50-500kHz). Cytotoxicity of TTFields was examined by cell counts, and intracellular accumulation of 7-aminoactinomycin D (7-AAD) was measured by flow cytometry. Exposing the cells to 7-AAD at different time points relative to TTFields application cessation was used to determine the kinetics of cell membrane permeability. The potential of TTFields to facilitate intracellular accumulation of anthracycline chemotherapeutics was tested in chemotherapy-sensitive and chemotherapy-resistant cells. RESULTS Elevated intracellular accumulation of 7-AAD was observed in all examined cell lines treated with TTFields, at an optimal frequency that differed from that for maximal TTFields-induced cytotoxicity. No intracellular accumulation of 7-AAD was seen for measurements performed after termination of TTFields application, indicating that increased cell membrane permeability by TTFields was temporary and reversible. Lastly, the accumulation of chemotherapeutic agents in chemotherapy-resistant cancer cells was elevated to the same extent as in matched chemotherapy-sensitive cells when TTFields were delivered concomitant with chemotherapy. CONCLUSIONS TTFields increased cancer cell permeability in a transient and reversible manner across multiple cancer cell types. The increased permeability enhanced intracellular accumulation of chemotherapeutics, even within chemotherapy-resistant cells.

EFFECT OF DICLOFOP METHYL AND 2,4-D ALONE AND IN COMBINATION ON LEAF CELL MEMBRANE PERMEABILITY OF WILD OATS AND BARLEY

1980 ◽  
Vol 60 (2) ◽  
pp. 773-775 ◽  
Author(s):  
NORA F. O’LEARY ◽  
J. T. O’DONOVAN ◽  
G. N. PRENDEVILLE
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Propanoic Acid ◽  
Leaf Cell ◽  
Wild Oats

Diclofop methyl (2-[4-(2,4-dichlorophenoxy)Phenoxy]) propanoic acid increased leaf cell membrane permeability of wild oats at 48 h and barley at 96 h after treatment while 2,4-D did not affect leaf cell membrane permeability in either species. A combination of both herbicides had a similar effect on leaf cell membrane permeability of wild oats as diclofop methyl alone. In barley, the combination increased leaf cell membrane permeability at 24 h after treatment compared to 96 h with diclofop methyl alone.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

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