scholarly journals Resistance to barley yellow dwarf luteovirus in Aegilops species

1994 ◽  
Vol 74 (3) ◽  
pp. 631-634 ◽  
Author(s):  
K. M. Makkouk ◽  
W. Ghulam ◽  
A. Comeau
Keyword(s):  
Triticum Aestivum ◽  
Agricultural Research ◽  
Sandwich Elisa ◽  
Yellow Dwarf ◽  
Aegilops Species ◽  
Elite Group ◽  
Barley Yellow Dwarf ◽  
Tissue Blot Immunoassay ◽  
Das Elisa ◽  
Barley Yellow Dwarf Luteovirus

One thousand and ninety-seven Aegilops accessions were evaluated for their reaction to a PAV serotype of barley yellow dwarf luteovirus (BYDV). The accessions tested belong to the species bicornis, biuncialis, caudata, crassa, columnaris, comosa, cylindrica, kotschyi, longissima, mutica, neglecta (= triaristata 4 ×), ovata, peregrina, searsii, sharonensis, speltoides, tauschii (= squarrosa), triuncialis, umbellulata, uniaristata, vavilovii and ventricosa. The first evaluation of virus levels in the different accessions was conducted at International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria, using double antibody sandwich ELISA (DAS-ELISA). Accession reaction ranged from highly resistant to highly susceptible. Thirty-eight Aegilops accessions resistant at ICARDA, were evaluated at Sainte-Foy, Quebec, Canada, by tissue-blot immunoassay. Diversity of response to BYDV infection was again observed in this elite group. Seven accessions belonging to the species biuncialis, caudata, neglecta and triuncialis were highly BYDV resistant at both locations; five of these originated from Bulgaria. Key words: Introgression, interspecific, Triticum aestivum, BYDV, ELISA, immunoassay, tissue blot

scholarly journals Serodetection of viruses associated to barley yellow dwarf (BYD) on cereals in Algeria

2005 ◽  
Vol 80 (3) ◽  
pp. 169-177 ◽  
Author(s):  
H. Belkahla ◽  
H. Lapierre
Keyword(s):  
Triticum Aestivum ◽  
Monoclonal Antibodies ◽  
Avena Sativa ◽  
Rhopalosiphum Padi ◽  
Schizaphis Graminum ◽  
Yellow Dwarf ◽  
Crop Season ◽  
Serological Tests ◽  
Barley Yellow Dwarf ◽  
Das Elisa

Surveys on viruses associated with Barley Yellow Dwarf (BYD) and their vectors were carried out in Algerian cereal areas (Guelma, Constantine, Algiers, Sidi-belabes, Adrar) in 1997 and 1998. Rhopalosiphum padi was present in all zones of culture, whereas R. maidis, Sitobion avenae, S. fragariae and Schizaphis graminum had only local distributions. In most areas BYD-like symptoms, i.e. dwarfing and yellowing of barley (Hordeum vulgare), dwarfing and reddening of oat (Avena sativa) and wheat (Triticum aestivum), were observed. Serological tests were done on these crops using DAS-ELISA (RMV and SGV) or TAS-ELISA using monoclonal antibodies specifie to CYDV-RPV or using different variant specifie BYDV-PAV (CpA and CpB) and BYDV-MAV monoclonal antibodies. BYDV-PAV was prevalent and few plant samples carrying RMV, SGV, BYDV-MAV or CYDV-RPV were detected. The relative frequencies of BYDV-PAV CpA and CpB serotypes were variable depending on the area and the crop season. The range of symptoms induced on barley by both Algerian BYDV-PAV CpB and BYDV-PAV CpA serotypes was mild to severe. Twenty-one BYDV-MAV isolates were compared using monoclonal antibodies, which distinguish two serotypes of this virus. Only one serotype was detected. This same serotype is also the most prevalent in Europe.

Cytological and molecular characterization of a Triticum aestivum × Lophopyrum ponticum backcross derivative resistant to barley yellow dwarf

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 876-881 ◽  
Author(s):  
Gan-Yuan Zhong ◽  
Patrick E. Mcguire ◽  
Calvin O. Qualset ◽  
Jan Dvořák
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Pairing ◽  
Barley Yellow Dwarf Virus ◽  
Virus Disease ◽  
Meiotic Chromosome ◽  
Yellow Dwarf ◽  
Homoeologous Chromosome ◽  
Barley Yellow Dwarf ◽  
Homoeologous Chromosome Pairing ◽  
Lophopyrum Ponticum

Barley yellow dwarf is the most damaging virus-caused disease in bread wheat (Triticum aestivum L.). A resistant line, SW335.1.2-13-11-1-5 (2n = 47), derived from a cross of T. aestivum × Lophopyrum ponticum was characterized by meiotic chromosome pairing, by in situ DNA hybridization and by expression of molecular markers to determine its chromosome constitution. All progeny of this line had three pairs of L. ponticum chromosomes from homoeologous chromosome groups 3, 5, and 6 and the 2n = 47 progeny had an additional L. ponticum monosome. The pairs from groups 3 and 6 were in the added state, while the group 5 pair was substituted for wheat chromosome 5D. Several wheat–wheat translocations with respect to the parental wheat genotype occurred in this line, presumably owing to the promotion of homoeologous chromosome pairing by L. ponticum chromosomes. It was hypothesized that homoeologous recombination results in homoeologous duplication–deletions in wheat chromosomes. An aberrant 3:1 disjunction creates the potential at each meiosis for replacement of these wheat chromosomes by homoeologous L. ponticum chromosomes. Wheat chromosomes 3A and 6A appeared to be in intermediate stages of this substitution process.Key words: wheat, wheatgrass, Lophopyrum, barley yellow dwarf virus, disease resistance, homoeologous chromosome recombination, homoeologous pairing, alien chromosome substitution.

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