scholarly journals ERRATUM: Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells

2009 ◽  
Vol 10 (4) ◽  
pp. 369
Author(s):  
Min-Soo Seo ◽  
Yun-Hyeok Jeong ◽  
Jeung-Ran Park ◽  
Sang-Bum Park ◽  
Kyoung-Hwan Rho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells

2009 ◽  
Vol 10 (3) ◽  
pp. 181 ◽  
Author(s):  
Min-Soo Seo ◽  
Yun-Hyeok Jeong ◽  
Jeung-Ran Park ◽  
Sang-Bum Park ◽  
Kyoung-Hwan Rho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Isolation And Characterization

Characterization of multipotent mesenchymal stem cells isolated from equine umbilical cord blood

2017 ◽  
Vol 43 (3) ◽  
pp. 262-265 ◽  
Author(s):  
D. G. Korovina ◽  
K. P. Yurov ◽  
S. V. Alexeenkova ◽  
E. A. Savchenkova ◽  
I. P. Savchenkova
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Multipotent Mesenchymal Stem Cells

Dramatic differences in activity of purines metabolizing ecto-enzymes between mesenchymal stem cells isolated from human umbilical cord blood and umbilical cord tissue

2013 ◽  
Vol 91 (6) ◽  
pp. 519-525 ◽  
Author(s):  
Katarzyna Roszek ◽  
Katarzyna Bomastek ◽  
Marian Drożdżal ◽  
Michał Komoszyński
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Proliferative Potential ◽  
Human Umbilical Cord ◽  
Isolation And Characterization ◽  
Umbilical Cord Tissue

The high quality human mesenchymal stem cells (MSCs) with remarkable expansion potential in culture are demonstrated to possess multifold clinical applications. However, their isolation and characterization are difficult and sometimes ambiguous. We exploited nucleotide metabolizing ecto-enzymes for more complete characterization of MSCs. Using standard methods of cell culturing and analyses, we detected significant differences in the activity of ecto-nucleotidases on the surface of MSCs isolated from umbilical cord tissue and MSC-like cells derived from umbilical cord blood. Interestingly, the proliferation rate and the immunophenotypic characteristics of mesenchymal stem cells also correspond to the activities of these enzymes. Compared with the CD90-, CD105-, and CD73-deficient and slowly proliferating UCB-MSC-like cells that had relatively higher ecto-NTPDases activity, the CD90-, CD105-, and CD73-positive and rapidly proliferating UC-MSCs rather had ecto-5′-nucleotidase activity and presented neither ecto-nucleotidases nor adenylate kinase activities. In summary, our results demonstrate for the first time that activity of purine nucleotide metabolizing ecto-enzymes differs significantly between mesenchymal stem cells drawn from different neonatal sources, corresponding with a distinct proliferative potential.

407: Characterization of fetal mesenchymal stem cells isolated from umbilical cord blood and amniotic fluid of the same donor

2009 ◽  
Vol 201 (6) ◽  
pp. S157
Author(s):  
Francesca Maria Russo ◽  
Patrizia Vergani ◽  
Francesca Gatto ◽  
Simona Marzorati ◽  
Ilaria Follesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood

2017 ◽  
Vol 37 (6) ◽  
pp. 643-649 ◽  
Author(s):  
Gabrielle R. Martins ◽  
Rebeca C. Marinho ◽  
Rosivaldo Q. Bezerra-Junior ◽  
Lilia M.C. Câmara ◽  
Luiz C. Albuquerque-Pinto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Active Role ◽  
Lineage Differentiation ◽  
Different Populations ◽  
Multipotent Mesenchymal Stem Cells

ABSTRACT: Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.

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