407: Characterization of fetal mesenchymal stem cells isolated from umbilical cord blood and amniotic fluid of the same donor

2009 ◽  
Vol 201 (6) ◽  
pp. S157
Author(s):  
Francesca Maria Russo ◽  
Patrizia Vergani ◽  
Francesca Gatto ◽  
Simona Marzorati ◽  
Ilaria Follesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Reproduction ◽  
2012 ◽  
Vol 143 (4) ◽  
pp. 455-468 ◽  
Author(s):  
Eleonora Iacono ◽  
Lara Brunori ◽  
Alessandro Pirrone ◽  
Pasquale Paolo Pagliaro ◽  
Francesca Ricci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Cord Blood ◽  
Cell Lines ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Wharton’S Jelly ◽  
Blue Staining ◽  
Wharton's Jelly

Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify and characterize stem cells from equine amniotic fluid (AF), CB and Wharton's Jelly (WJ). Samples were obtained from 13 mares at labour. AF and CB cells were isolated by centrifugation, while WJ was prepared by incubating with an enzymatic solution for 2 h. All cell lines were cultured in DMEM/TCM199 plus fetal bovine serum. Fibroblast-like cells were observed in 7/10 (70%) AF, 6/8 (75%) CB and 8/12 (66.7%) WJ samples. Statistically significant differences were found between cell-doubling times (DTs): cells isolated from WJ expanded more rapidly (2.0±0.6 days) than those isolated from CB (2.6±1.3 days) and AF (2.3±1.0 days) (P<0.05). Positive von Kossa and Alizarin Red S staining confirmed osteogenesis. Alcian Blue staining of matrix glycosaminoglycans illustrated chondrogenesis and positive Oil Red O lipid droplets staining suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105; and negative for CD34, CD14 and CD45. These findings suggest that equine MSCs from AF, UCB and WJ appeared to be a readily obtainable and highly proliferative cell lines from a uninvasive source that may represent a good model system for stem cell biology and cellular therapy applications in horses. However, to assess their use as an allogenic cell source, further studies are needed for evaluating the expression of markers related to cell immunogenicity.

Characterization of multipotent mesenchymal stem cells isolated from equine umbilical cord blood

2017 ◽  
Vol 43 (3) ◽  
pp. 262-265 ◽  
Author(s):  
D. G. Korovina ◽  
K. P. Yurov ◽  
S. V. Alexeenkova ◽  
E. A. Savchenkova ◽  
I. P. Savchenkova
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells

2009 ◽  
Vol 10 (3) ◽  
pp. 181 ◽  
Author(s):  
Min-Soo Seo ◽  
Yun-Hyeok Jeong ◽  
Jeung-Ran Park ◽  
Sang-Bum Park ◽  
Kyoung-Hwan Rho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Isolation And Characterization

scholarly journals ERRATUM: Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells

2009 ◽  
Vol 10 (4) ◽  
pp. 369
Author(s):  
Min-Soo Seo ◽  
Yun-Hyeok Jeong ◽  
Jeung-Ran Park ◽  
Sang-Bum Park ◽  
Kyoung-Hwan Rho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Isolation And Characterization

scholarly journals Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood

2017 ◽  
Vol 37 (6) ◽  
pp. 643-649 ◽  
Author(s):  
Gabrielle R. Martins ◽  
Rebeca C. Marinho ◽  
Rosivaldo Q. Bezerra-Junior ◽  
Lilia M.C. Câmara ◽  
Luiz C. Albuquerque-Pinto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Active Role ◽  
Lineage Differentiation ◽  
Different Populations ◽  
Multipotent Mesenchymal Stem Cells

ABSTRACT: Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.

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