Rapid in vitro Multiplication, Callogenesis and Indirect Shoot Regeneration in Ipomoea Mauritiana – A Rare Medicinal Plant in Bangladesh

2013 ◽  
Vol 02 (06) ◽  
Author(s):  
M S Islam
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
In Vitro Multiplication

scholarly journals Efficient Micropropagation of Highly Economic, Medicinal and Ornamental PlantLallemantia iberica(Bieb.) Fisch. and C. A. Mey

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Fethi Ahmet Ozdemir ◽  
Mehmet Ugur Yildirim ◽  
Mahsa Pourali Kahriz
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Growth Chamber ◽  
Ms Medium ◽  
Regenerated Plantlets

Lallemantia iberica(Bieb.) Fisch. and C. A. Mey is high valued annual ornamental and medicinal plant from Lamiaceae family that prefers dry sunny hillsides, roadsides, slopes, and fallow fields over an altitude of 500–2150 m. It bears beautiful white flowers and bloom from April to June each year. This study reportsL. ibericamicropropagation using cotyledon node explants isolated from 15-day-oldin vitroregenerated plantlets. The cotyledon node explants were cultured on MS medium containing 0.50, 1.00 plus 2.00 mg/L BAP, 0.00, 0.01, and 0.02 mg/L NAA. Maximum shoot regeneration was noted on MS medium containing 0.50 mg/L BAP. Well-developed micropropagated shoots were rooted on MS medium containing 1.00 mg/L IBA. The rooted plants were easily hardened in the growth chamber and acclimatised in greenhouse.

scholarly journals Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

2021 ◽  
Vol 12 ◽  
Author(s):  
Denis Okello ◽  
Sungyu Yang ◽  
Richard Komakech ◽  
Yuseong Chung ◽  
Endang Rahmat ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Developmental Stages ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration Capacity ◽  
Root Explants ◽  
Indirect Regeneration

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

scholarly journals Conservation of the endangered medicinal plant Picrorhiza kurroa through in vitro multiple shoot regeneration

2019 ◽  
Vol 100 (3) ◽  
pp. 209-217
Author(s):  
Varnika Chaudhary ◽  
Shalini Singh ◽  
Rohit Sharma ◽  
Ajay Singh ◽  
Nishesh Sharma
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Picrorhiza Kurroa ◽  
Endangered Medicinal Plant ◽  
Multiple Shoot Regeneration

In vitro multiplication, genetic fidelity and phytochemical potentials of Vaccinium arctostaphylos L.: An endangered medicinal plant

2019 ◽  
Vol 141 ◽  
pp. 111812 ◽  
Author(s):  
Mehdi Bakhshipour ◽  
Mohammad Mafakheri ◽  
Mojtaba Kordrostami ◽  
Ali Zakir ◽  
Najmeh Rahimi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Fidelity ◽  
In Vitro Multiplication ◽  
Endangered Medicinal Plant

scholarly journals In vitro shoot regeneration of Mas Cotek (Ficus deltoidea Jack) A valuable Malay Medicinal Plant

2015 ◽  
Vol 3 (3) ◽  
pp. 46-50
Author(s):  
Ilfah Husna Abdullah Ripain ◽  
Paritala Vikram ◽  
Fatimah Kayat ◽  
Dwi Susanto ◽  
Mohammed Aurifullah
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Clonal Propagation ◽  
Ficus Deltoidea ◽  
Economic Area ◽  
Over Exploitation ◽  
Day By Day ◽  
In Vitro Shoot Regeneration

Mas Cotek (Ficus deltoidea) is one of the famous Malay medicinal plants and it is categorized as a priority herb under Entry Point Project 1 (EPP1) of National Key Economic Area (NKEA) Agriculture. Different parts of the plant had been used traditionally to treat various ailments. Due to over-exploitation, the population of many medicinal plants in the nature including Mas Cotek is decreasing day by day. This causes a big challenge to the pharmaceutical and herbal industries as they will run out of the source of plant material to support the demand from the consumers. Hence the present study aims to produce a protocol on shoot regeneration of Mas Cotek by using plant tissue culture. Mature nodes of the plant were aseptically cultured onto Murashige and Skoog (MS) media supplemented with different concentrations and combinations of plant growth regulators like 1.0-3.0 mg/l Kinetin, 1.0-3.0 mg/l BAP, 1.0-3.0 mg/l Kinetin+0.5-1.5 mg/l NAA and 1.0-3.0 mg/l BAP+0.5-1.5 mg/l NAA. 3.0 mg/l BAP wasthe best medium for shoot regeneration of Mas Cotek as it can produce more shoots eitherwhen used singly or in combination with NAA respectively. For the fastest shoot regeneration, MS media with 1.0 mg/l BAP + 0.5 mg/l NAA was more favourable since it can produce shoot within 4 weeks. The protocols developed under this study are highly useful for the mass clonal propagation of Mas Cotek as well as to conserve this valuable medicinal plant for future use.

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