scholarly journals In vitro shoot regeneration of Mas Cotek (Ficus deltoidea Jack) A valuable Malay Medicinal Plant

2015 ◽  
Vol 3 (3) ◽  
pp. 46-50
Author(s):  
Ilfah Husna Abdullah Ripain ◽  
Paritala Vikram ◽  
Fatimah Kayat ◽  
Dwi Susanto ◽  
Mohammed Aurifullah
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Clonal Propagation ◽  
Ficus Deltoidea ◽  
Economic Area ◽  
Over Exploitation ◽  
Day By Day ◽  
In Vitro Shoot Regeneration

Mas Cotek (Ficus deltoidea) is one of the famous Malay medicinal plants and it is categorized as a priority herb under Entry Point Project 1 (EPP1) of National Key Economic Area (NKEA) Agriculture. Different parts of the plant had been used traditionally to treat various ailments. Due to over-exploitation, the population of many medicinal plants in the nature including Mas Cotek is decreasing day by day. This causes a big challenge to the pharmaceutical and herbal industries as they will run out of the source of plant material to support the demand from the consumers. Hence the present study aims to produce a protocol on shoot regeneration of Mas Cotek by using plant tissue culture. Mature nodes of the plant were aseptically cultured onto Murashige and Skoog (MS) media supplemented with different concentrations and combinations of plant growth regulators like 1.0-3.0 mg/l Kinetin, 1.0-3.0 mg/l BAP, 1.0-3.0 mg/l Kinetin+0.5-1.5 mg/l NAA and 1.0-3.0 mg/l BAP+0.5-1.5 mg/l NAA. 3.0 mg/l BAP wasthe best medium for shoot regeneration of Mas Cotek as it can produce more shoots eitherwhen used singly or in combination with NAA respectively. For the fastest shoot regeneration, MS media with 1.0 mg/l BAP + 0.5 mg/l NAA was more favourable since it can produce shoot within 4 weeks. The protocols developed under this study are highly useful for the mass clonal propagation of Mas Cotek as well as to conserve this valuable medicinal plant for future use.

scholarly journals IN VITRO SHOOT REGENERATION OF SWALLOW ROOT (DECALEPIS HAMILTONII) – A STENO-ENDEMIC RED LISTED MEDICINAL PLANT

2020 ◽  
pp. 188-191
Author(s):  
Manjula Ranganatha ◽  
Annapurna AS ◽  
Ashwani Sharma ◽  
Nagashree N Rao
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Nodal Segments ◽  
Direct Regeneration ◽  
Multiplication Rate ◽  
Decalepis Hamiltonii ◽  
Shoot Number ◽  
In Vitro Shoot Regeneration

Objective: In vitro shoot regeneration of Decalepis hamiltonii Wight and Arn. is an endangered endemic medicinal plant using biotechnological interventions and to conserve this threatened species. Methods: In the present study, various explants such as shoot tip, leaf, and nodal segments were inoculated on Murashige and Skoog media augmented with different hormonal regimes of auxin and cytokinin combinations, namely, naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), benzyl adenine (BAP), 6-(γ,γ-Dimethylallylamino)purine (2iP), and triacontanol (TRIA). Results: Direct regeneration of shoots obtained in 3.0 mg/l 2iP alone and in combination with 0.1 mg/l IAA and/or 1.0 mg/l BAP exhibited the best response with average shootlet length being 6.5±0.17–8.0±0.92 cm, respectively, and percentage response was between 68% and 75%. The callus induced regeneration was obtained from both nodal and leaf explants with maximum response (85%) observed in combination of (2.0 mg/l) 2iP, (1.0 mg/l) IAA and (2.0 mg/l) kinetin with multiple shoots showing mean shoot number of 1.83 and average shootlet length of 6.3±0.19 cm. Conclusions: The current research provides a competent in vitro propagation method for Decalepis which could be commercialized for developing identical plants with good mass multiplication rate and for better conservation of the germplasm.

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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