scholarly journals A fast and simple method for the polymerase chain reaction-based sexing of livestock embryos

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
K.C.S. Tavares ◽  
I.S. Carneiro ◽  
D.B. Rios ◽  
C. Feltrin ◽  
A.K.C. Ribeiro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Simple Method ◽  
Polymerase Chain

scholarly journals A simple method for site-directed mutagenesis using the polymerase chain reaction

1989 ◽  
Vol 17 (16) ◽  
pp. 6545-6551 ◽  
Author(s):  
Anne Hemsley ◽  
Norman Arnheim ◽  
Michael Dennis Toney ◽  
Gino Cortopassi ◽  
David J. Galas
Keyword(s):  
Polymerase Chain Reaction ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Simple Method ◽  
Polymerase Chain

A simple method for detecting human polyomavirus DNA in urine by the polymerase chain reaction

1996 ◽  
Vol 58 (1-2) ◽  
pp. 131-136 ◽  
Author(s):  
Deching Chang ◽  
Meilin Wang ◽  
Wei-chih Ou ◽  
Rong-tai Tsai ◽  
Chiung-yau Fung ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Polyomavirus ◽  
Chain Reaction ◽  
Simple Method ◽  
Polymerase Chain

scholarly journals Specific Polymerase Chain Reaction Primers for the Detection of Plasmodiophora brassicae in Soil and Water

1999 ◽  
Vol 89 (5) ◽  
pp. 392-397 ◽  
Author(s):  
R. Faggian ◽  
S. R. Bulman ◽  
A. C. Lawrie ◽  
I. J. Porter
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodiophora Brassicae ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Simple Method ◽  
Soil P ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Primers ◽  
Soil And Water

The development of specific oligonucleotide primers for Plasmodiophora brassicae has led to a nested polymerase chain reaction (PCR) detection method for P. brassicae in soil and water. Initially, the PCR was used to amplify a section of the rDNA repeat. The PCR products were sequenced and the data used to design primers that were directed at the ribosomal RNA genes and internal transcribed spacer regions. Specificity was tested against more than 40 common soil organisms, host plants, and spore suspension contaminants, as well as P. brassicae isolates from around Australia and the world. Sensitivity was determined to be 0.1 fentograms (fg; 10-15 g) for pure template and as low as 1,000 spores per g of potting mix. In soil, P. brassicae was detected in all soils where the inoculum was sufficient to result in clubroot symptoms. Also outlined is a simple method of DNA extraction from soil.

Determination of gender in cetaceans by the polymerase chain reaction

1992 ◽  
Vol 70 (11) ◽  
pp. 2166-2170 ◽  
Author(s):  
Per J. Palsbøll ◽  
Anna Vader ◽  
Ingrid Bakke ◽  
M. Raafat El-Gewely
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Chromosome ◽  
Chain Reaction ◽  
Cetacean Species ◽  
Simple Method ◽  
Fin Whale ◽  
Skin Biopsies ◽  
Polymerase Chain ◽  
Free Ranging

We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFY/ZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore allows gender to be determined from skin biopsies taken from free-ranging specimens. In the fin whale, Balaenoptera physalus, no gender-specific bands were observed when the ZFY/ZFX system was used, but when the SRY system was used, sex was accurately determined. Previous studies in other mammals have also shown the SRY system to be more reliable in sex determination. We therefore recommend amplification of the SRY region alone or in parallel with the ZFY/ZFX regions, as described here, as a test for gender in cetaceans and other mammals.

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