scholarly journals Detection and quantification of toxins in cultures of microcystis aeruginosa (pcc 7820) by hplc and protein phosphatase inhibition assayffect of blending various collectors at bulk

2010 ◽  
Vol 6 (1) ◽  
Author(s):  
G.A Akin-Oriola ◽  
L.A Lawton
Keyword(s):  
Microcystis Aeruginosa ◽  
Protein Phosphatase ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition ◽  
Detection And Quantification

Optimization of intracellular microcystin-LR extraction for its analysis by protein phosphatase inhibition assay

2009 ◽  
Vol 60 (7) ◽  
pp. 1903-1909 ◽  
Author(s):  
E. Sevilla ◽  
H. Smienk ◽  
P. Razquin ◽  
L. Mata ◽  
M. L. Peleato
Keyword(s):  
Microcystis Aeruginosa ◽  
Trifluoroacetic Acid ◽  
Water Samples ◽  
Protein Phosphatase ◽  
Screening Method ◽  
Tween 20 ◽  
Inhibition Assay ◽  
Protein Phosphatase Inhibition ◽  
Extraction Volume ◽  
Phosphatase Inhibition

Microcystins are toxins produced by some strains of cyanobacteria. Several methods have been developed to allow the quantification of microcystins, which are mainly endotoxins. Among those methods, the protein phosphatase inhibition assay is a good candidate as a screening method because of its sensitivity, simplicity and specificity. In this work a method for intracellular microcystin extraction in field water samples and lab cyanobacterial cultures prior to their analysis by protein phosphatase inhibition assay has been optimized. Microcystin-LR and Microcystis aeruginosa PCC 7806 were used as reference microcystin and strain, respectively, in order to optimize the protocol. The protocol consists on filtering the sample through a nylon filter of 0.8 μm, filter extraction with methanol 80% 0.1% trifluoroacetic acid (TFA) 0.1% tween 20, extract centrifugation and supernatant dilution (1/20). The establishment of an extraction protocol was carried out determining the extraction volume, time of extraction and number of extractions. The advantages of the method developed in this work are basically its simplicity and avoiding the use of specific and expensive equipment.

scholarly journals Model-driven discovery of calcium-related protein-phosphatase inhibition in plant guard cell signaling

2019 ◽  
Vol 15 (10) ◽  
pp. e1007429 ◽  
Author(s):  
Parul Maheshwari ◽  
Hao Du ◽  
Jen Sheen ◽  
Sarah M. Assmann ◽  
Reka Albert
Keyword(s):  
Cell Signaling ◽  
Guard Cell ◽  
Protein Phosphatase ◽  
Related Protein ◽  
Model Driven ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition

Okadaic Acid in New Zealand Sponges: Detection by Cytotoxicity, Protein Phosphatase Inhibition and Immunoassay techniques

1998 ◽  
Vol 11 (4) ◽  
pp. 305-312 ◽  
Author(s):  
Nigel B. Perry ◽  
Gill Ellis ◽  
John W. Blunt ◽  
Timothy A. J. Haystead ◽  
Robin J. Lake ◽  
...  
Keyword(s):  
New Zealand ◽  
Okadaic Acid ◽  
Protein Phosphatase ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition

scholarly journals Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins

Toxicon ◽  
2006 ◽  
Vol 47 (7) ◽  
pp. 742-746 ◽  
Author(s):  
Yi-Min Chen ◽  
Tzong-Huei Lee ◽  
Shyh-Jye Lee ◽  
Hsien-Bin Huang ◽  
Rang Huang ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition
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