Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay

1994 ◽  
Vol 28 (4) ◽  
pp. 871-876 ◽  
Author(s):  
G JONES
Keyword(s):  
Microcystis Aeruginosa ◽  
Protein Phosphatase ◽  
Inhibition Assay ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition ◽  
Recreational Lake

Optimization of intracellular microcystin-LR extraction for its analysis by protein phosphatase inhibition assay

2009 ◽  
Vol 60 (7) ◽  
pp. 1903-1909 ◽  
Author(s):  
E. Sevilla ◽  
H. Smienk ◽  
P. Razquin ◽  
L. Mata ◽  
M. L. Peleato
Keyword(s):  
Microcystis Aeruginosa ◽  
Trifluoroacetic Acid ◽  
Water Samples ◽  
Protein Phosphatase ◽  
Screening Method ◽  
Tween 20 ◽  
Inhibition Assay ◽  
Protein Phosphatase Inhibition ◽  
Extraction Volume ◽  
Phosphatase Inhibition

Microcystins are toxins produced by some strains of cyanobacteria. Several methods have been developed to allow the quantification of microcystins, which are mainly endotoxins. Among those methods, the protein phosphatase inhibition assay is a good candidate as a screening method because of its sensitivity, simplicity and specificity. In this work a method for intracellular microcystin extraction in field water samples and lab cyanobacterial cultures prior to their analysis by protein phosphatase inhibition assay has been optimized. Microcystin-LR and Microcystis aeruginosa PCC 7806 were used as reference microcystin and strain, respectively, in order to optimize the protocol. The protocol consists on filtering the sample through a nylon filter of 0.8 μm, filter extraction with methanol 80% 0.1% trifluoroacetic acid (TFA) 0.1% tween 20, extract centrifugation and supernatant dilution (1/20). The establishment of an extraction protocol was carried out determining the extraction volume, time of extraction and number of extractions. The advantages of the method developed in this work are basically its simplicity and avoiding the use of specific and expensive equipment.

scholarly journals Comparison of Protein Phosphatase Inhibition Assay with LC-MS/MS for Diagnosis of Microcystin Toxicosis in Veterinary Cases

Marine Drugs ◽  
2016 ◽  
Vol 14 (3) ◽  
pp. 54 ◽  
Author(s):  
Caroline Moore ◽  
Jeanette Juan ◽  
Yanping Lin ◽  
Cynthia Gaskill ◽  
Birgit Puschner
Keyword(s):  
Protein Phosphatase ◽  
Inhibition Assay ◽  
Protein Phosphatase Inhibition ◽  
Phosphatase Inhibition
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