Effect of Size and Source of Shoot Tips on Psorosis-A and Exocortis Content of Navel Orange Plants Obtained by Shoot-Tip Grafting in vitro

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

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Citrus Shoot Tip Grafting in Vitro

Biotechnology in Agriculture and Forestry - High-Tech and Micropropagation II ◽

10.1007/978-3-642-76422-6_17 ◽

1992 ◽

pp. 327-338 ◽

Cited By ~ 7

Author(s):

L. Navarro

Keyword(s):

Shoot Tip ◽

Shoot Tip Grafting

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Shoot-tip grafting in vitro: impact in the citrus industry and research applications.

Citrus genetics, breeding and biotechnology ◽

10.1079/9780851990194.0353 ◽

2009 ◽

pp. 353-364 ◽

Cited By ~ 11

Author(s):

L. Navarro ◽

J. Juárez

Keyword(s):

Shoot Tip ◽

Citrus Industry ◽

Shoot Tip Grafting

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Separation of citrus viroids by shoot-tip grafting in vitro

Plant Pathology ◽

10.1111/j.1365-3059.1990.tb02523.x ◽

1990 ◽

Vol 39 (3) ◽

pp. 472-476 ◽

Cited By ~ 2

Author(s):

J. JUÁREZ ◽

M. I. MOLINS ◽

L. NAVARRO ◽

N. DURAN-VILA

Keyword(s):

Shoot Tip ◽

Shoot Tip Grafting ◽

Citrus Viroids

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In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

Journal of Bio-Science ◽

10.3329/jbs.v17i0.7122 ◽

1970 ◽

Vol 17 ◽

pp. 139-144 ◽

Cited By ~ 1

Author(s):

MS Rahman ◽

MF Hasan ◽

R Das ◽

MS Hossain ◽

M Rahman

Keyword(s):

Multiple Shoots ◽

Shoot Elongation ◽

Shoot Formation ◽

Multiple Shoot ◽

Shoot Tips ◽

Shoot Tip ◽

Root Induction ◽

Ms Medium ◽

Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

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An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

Plants ◽

10.3390/plants9020235 ◽

2020 ◽

Vol 9 (2) ◽

pp. 235 ◽

Cited By ~ 1

Author(s):

Kyungtae Park ◽

Bo Kook Jang ◽

Ha Min Lee ◽

Ju Sung Cho ◽

Cheol Hee Lee

Keyword(s):

Nitrogen Sources ◽

Culture Conditions ◽

Shoot Tips ◽

Shoot Tip ◽

Soil Conditions ◽

Ms Medium ◽

Ex Vitro ◽

Shoot Tip Culture ◽

Propagation Methods

Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.

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