Improved in vitro propagation of Rose cultivars against varying concentrations of carbon source, agar and plant growth regulators

2015 ◽  
Vol 22 (2) ◽  
pp. 69-73
Author(s):  
Kumud Saklani ◽  
Hem Pant ◽  
Vinod Bisht ◽  
Arun Singh ◽  
Vijay Rawat
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Half Strength ◽  
High Sucrose

The present study was conducted to improve upon the micropropagation protocol of Rose cultivars by modification of the MS medium through variations in sucrose and agar concentrations thereby enhancing the shoot multiplication and rooting efficiency of the nodal explants. High sucrose concentrations and low agar concentrations favoured shoot mutiplication during the in vitro stages. Enhanced multiplcation and growth was observed on sub culturing the mother explants with regenerated shoots on fresh MS medium containing sucrose (3.5%, w/v) and agar (0.6%, w/v), supplemented with lower concentrations cytokinin combination of BAP and KN (2.5 mgl-1+1.5 mgl-1) respectively. Half strength MS medium containing sucrose (2.0%, w/v) and agar (0.3%, w/v) with NAA and BAP (2.0 mgl-1+0.5 mgl-1) in combination was most effective for rooting.

scholarly journals In vitro propagation of Gerbera (Gerbera jamesonii Bolus)

2013 ◽  
Vol 7 (1) ◽  
pp. 50-58
Author(s):  
Sattar Abdullah Shlahi ◽  
Duha Mysire Majeed ◽  
Salah Mohammed Hasan
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Ms Medium ◽  
Gerbera Jamesonii

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

2019 ◽  
Vol 47 (3) ◽  
pp. 987-994
Author(s):  
Joon-Ho KWON ◽  
Young-Sik PARK ◽  
Si-Hong KIM ◽  
Jae-Yun HEO
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Vitis Amurensis ◽  
Root Induction ◽  
Propagation Methods

Vitis amurensis ‘Cheongsan’ is a plant with high economic value in both medical and agricultural applications. However, its utilization has been restricted owing to difficulties encountered when applying traditional mass propagation methods, requiring instead application of in vitro propagation methods for their mass scale production. Hence, this study was conducted to find the optimal plant growth regulators for shoot multiplication and root induction during in vitro propagation. Among the three cytokinins used at multiple concentrations for culture initiation and shoot multiplication, the most positive response was found with MS medium containing 5.0 μM 6-benzyladenine (BA), compared to more modest responses from other types of cytokinin, such as kinetin (KIN) and thidiazuron (TDZ). For root induction, medium supplemented with α-naphthaleneacetic acid (NAA) produced a callus and inhibited shoot growth in explants, whereas indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) did not create any significant problems, but did display differences in root induction efficiencies. Generally, root induction responses with IBA were better that those with IAA. The maximum rooting rates were observed without callus formation and no shoot growth inhibition from explants grown on media supplemented with 0.67 μM IBA. Further, inter-simple sequence repeat (ISSR) analyses revealed that micropropagated plantlets generated in medium supplemented with 5.0 μM BA and 0.67 μM IBA did not lead to genetic variation. Therefore, the application of the in vitro propagation method developed in this study could be used on a commercial scale and will offer opportunities to strengthen the industrial use of V. amurensis ‘Cheongsan’.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea

2018 ◽  
Vol 75 (2) ◽  
pp. 134
Author(s):  
Ileana MICLEA ◽  
Rita BERNAT
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Multiplication ◽  
Time Frame ◽  
Naphthaleneacetic Acid ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Frame Number

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.

scholarly journals In vitro propagation of Althaea officinalis : the role of plant growth regulators in morphogenesis

2017 ◽  
Vol 98 (3) ◽  
pp. 167-173
Author(s):  
A. Mujib ◽  
Tanu Pipal ◽  
Muzamil Ali ◽  
Dipti Tonk ◽  
Nadia Zafar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Althaea Officinalis
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