scholarly journals Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

2019 ◽  
Vol 47 (3) ◽  
pp. 987-994
Author(s):  
Joon-Ho KWON ◽  
Young-Sik PARK ◽  
Si-Hong KIM ◽  
Jae-Yun HEO
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Vitis Amurensis ◽  
Root Induction ◽  
Propagation Methods

Vitis amurensis ‘Cheongsan’ is a plant with high economic value in both medical and agricultural applications. However, its utilization has been restricted owing to difficulties encountered when applying traditional mass propagation methods, requiring instead application of in vitro propagation methods for their mass scale production. Hence, this study was conducted to find the optimal plant growth regulators for shoot multiplication and root induction during in vitro propagation. Among the three cytokinins used at multiple concentrations for culture initiation and shoot multiplication, the most positive response was found with MS medium containing 5.0 μM 6-benzyladenine (BA), compared to more modest responses from other types of cytokinin, such as kinetin (KIN) and thidiazuron (TDZ). For root induction, medium supplemented with α-naphthaleneacetic acid (NAA) produced a callus and inhibited shoot growth in explants, whereas indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) did not create any significant problems, but did display differences in root induction efficiencies. Generally, root induction responses with IBA were better that those with IAA. The maximum rooting rates were observed without callus formation and no shoot growth inhibition from explants grown on media supplemented with 0.67 μM IBA. Further, inter-simple sequence repeat (ISSR) analyses revealed that micropropagated plantlets generated in medium supplemented with 5.0 μM BA and 0.67 μM IBA did not lead to genetic variation. Therefore, the application of the in vitro propagation method developed in this study could be used on a commercial scale and will offer opportunities to strengthen the industrial use of V. amurensis ‘Cheongsan’.

scholarly journals In vitro propagation of Gerbera (Gerbera jamesonii Bolus)

2013 ◽  
Vol 7 (1) ◽  
pp. 50-58
Author(s):  
Sattar Abdullah Shlahi ◽  
Duha Mysire Majeed ◽  
Salah Mohammed Hasan
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Ms Medium ◽  
Gerbera Jamesonii

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

scholarly journals STANDARDIZATION THE EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO PROPAGATION OF BANANA (MUSA SPP) EXPLANTS: A REVIEW

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Kothula Pravalika
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Musa Spp ◽  
The Family ◽  
Micro Propagation ◽  
Propagation Methods ◽  
Herbaceous Perennial

Banana (Musa spp.) is a large herbaceous perennial monocotyledonous and monocarpic plant. It belongs to the family Musaceae. Banana is one of the popular berry fruit which is available to every common person. Banana crop is mostly cultivated through sword suckers, for ease production some in vitro propagation methods came into existence like tissue culture technology. Some of them are micro propagation, clonal propagation etc, For in vitro growth of the excited tissues, plant growth regulators have been considered as one of the most significant factor. Most commonly, auxins and cytokinins are used in the growth and development of the banana explants. The plant growth regulators are required in different concentrations along with Murashige and Skoog media for the best shooting and rooting of the explants.

Improved in vitro propagation of Rose cultivars against varying concentrations of carbon source, agar and plant growth regulators

2015 ◽  
Vol 22 (2) ◽  
pp. 69-73
Author(s):  
Kumud Saklani ◽  
Hem Pant ◽  
Vinod Bisht ◽  
Arun Singh ◽  
Vijay Rawat
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Half Strength ◽  
High Sucrose

The present study was conducted to improve upon the micropropagation protocol of Rose cultivars by modification of the MS medium through variations in sucrose and agar concentrations thereby enhancing the shoot multiplication and rooting efficiency of the nodal explants. High sucrose concentrations and low agar concentrations favoured shoot mutiplication during the in vitro stages. Enhanced multiplcation and growth was observed on sub culturing the mother explants with regenerated shoots on fresh MS medium containing sucrose (3.5%, w/v) and agar (0.6%, w/v), supplemented with lower concentrations cytokinin combination of BAP and KN (2.5 mgl-1+1.5 mgl-1) respectively. Half strength MS medium containing sucrose (2.0%, w/v) and agar (0.3%, w/v) with NAA and BAP (2.0 mgl-1+0.5 mgl-1) in combination was most effective for rooting.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea

2018 ◽  
Vol 75 (2) ◽  
pp. 134
Author(s):  
Ileana MICLEA ◽  
Rita BERNAT
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Multiplication ◽  
Time Frame ◽  
Naphthaleneacetic Acid ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Frame Number

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.

scholarly journals In vitro propagation of Althaea officinalis : the role of plant growth regulators in morphogenesis

2017 ◽  
Vol 98 (3) ◽  
pp. 167-173
Author(s):  
A. Mujib ◽  
Tanu Pipal ◽  
Muzamil Ali ◽  
Dipti Tonk ◽  
Nadia Zafar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Althaea Officinalis
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