AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.
Characterization of Acetylxylan Esterase from White-Rot Fungus Irpex lacteus