Evaluation of Fluorescence Polarization Immunoassay with Monoclonal Antibody for Determination of Ciclosporin in Whole Blood of Partial Liver Transplant Recipients.

Abstract In a prospective study we evaluated a novel fluorescence polarization immunoassay (FPIA) for determining cyclosporine (CsA) in whole blood. FPIA uses a monoclonal antibody and is performed on the TDx (Abbott). The within-series (CV less than 2%) and between-days (CV less than 3.3%) precision of the assay was excellent. The results obtained by the monoclonal FPIA in samples from transplant patients (n = 100) averaged 31.9% and 20.2% higher than those by HPLC and a specific radioimmunoassay (INCStar), respectively. Results by all three methods correlated well. Follow-up studies during the early course after liver transplantation, however, suggested that high metabolite concentrations affect FPIA results. This is explained by previously described cross-reactions of the monoclonal antibody with some CsA metabolites. The FPIA results in samples of such patients should be interpreted cautiously.

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Epstein-Barr virus DNA monitoring in serum and whole blood in pediatric liver transplant recipients who do or do not discontinue immunosuppressive therapy

Pediatric Transplantation ◽

10.1111/petr.12875 ◽

2016 ◽

Vol 21 (5) ◽

pp. e12875 ◽

Cited By ~ 3

Author(s):

C. Kullberg-Lindh ◽

R. Saalman ◽

M. Olausson ◽

G. Herlenius ◽

M. Lindh

Keyword(s):

Liver Transplant ◽

Immunosuppressive Therapy ◽

Whole Blood ◽

Epstein Barr Virus ◽

Transplant Recipients ◽

Barr Virus ◽

Pediatric Liver ◽

Epstein Barr ◽

Liver Transplant Recipients ◽

Pediatric Liver Transplant

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Immunoprophylaxis with basiliximab, a chimeric anti[ndash ]interleukin-2 receptor monoclonal antibody, in combination with azathioprine-containing triple therapy in liver transplant recipients

Liver Transplantation ◽

10.1053/jlts.2002.30882 ◽

2002 ◽

Vol 8 (2) ◽

pp. 123-131 ◽

Cited By ~ 71

Author(s):

Y Calmus

Keyword(s):

Monoclonal Antibody ◽

Liver Transplant ◽

Triple Therapy ◽

Interleukin 2 ◽

Transplant Recipients ◽

Interleukin 2 Receptor ◽

Liver Transplant Recipients ◽

Receptor Monoclonal Antibody

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Measurement of Cyclosporine by Liquid Chromatography and Three Immunoassays in Blood from Liver, Cardiac, and Renal Transplant Recipients

Clinical Chemistry ◽

10.1093/clinchem/38.11.2300 ◽

1992 ◽

Vol 38 (11) ◽

pp. 2300-2306 ◽

Cited By ~ 25

Author(s):

J H McBride ◽

S S Kim ◽

D O Rodgerson ◽

A F Reyes ◽

M K Ota

Keyword(s):

Renal Transplant ◽

Renal Transplant Recipients ◽

Positive Bias ◽

Transplant Recipients ◽

Fluorescence Polarization Immunoassay ◽

Immunoassay Technique ◽

Pediatric Renal Transplant ◽

Pediatric Renal Transplant Recipients ◽

Liver Transplant Recipients

Abstract In an effort to replace HPLC for whole-blood determination of cyclosporine (CsA), we compared HPLC with radioimmunoassay (RIA; INCSTAR, Cyclo-Trac SP assay), fluorescence polarization immunoassay (FPIA; Abbott TDx), and in-house modified enzyme-multiplied immunoassay technique (EMIT; Syva Co.). For blood samples from 200 various transplant recipients, RIA = 1.262 (HPLC) - 8.16, r = 0.983; FPIA = 1.200 (HPLC) + 19.90, r = 0.981; and EMIT = 1.038 (HPLC) + 11.28, r = 0.985. For segregation by transplant type, RIA, FPIA, and EMIT demonstrated positive biases of 27%, 12%, and 3%, respectively, for liver transplant recipients (n = 50) when compared with HPLC. Heart transplant recipients (n = 50) gave positive bias values of 23%, 14%, and 4% for RIA, FPIA, and EMIT, respectively. Adult renal transplant recipients (n = 50) demonstrated positive bias values of 30%, 31%, and 0% for RIA, FPIA, and EMIT, respectively. For pediatric renal transplant recipients (n = 50), positive biases of 40%, 31%, and 9% were obtained for RIA, FPIA, and EMIT, respectively. We conclude that the modified EMIT represents the best replacement for HPLC.

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