Sex Determination of the Japanese Serow (Capricornis crispus) by Fecal DNA Analysis

Random amplified polymorphic DNA (RAPD) technique with male associated decamer primer S1478 was used to amplify DNA from 72 leaf samples collected from Ginkgo biloba trees with known sexual determinism in the canton of Geneva, Switzerland. This marker was found to be male-specific and was lacking in all female plants. Automated random polymorphic DNA analysis (ARPA), a new automated technology developed in the frame of this work, proved highly effective in distinguishing males and females with 100% efficiency and successful in male and female discrimination from a collection of young seedlings derived from a sexual cross. Our findings provide unambiguous evidence that ARPA combined with the male-associated decamer primer S1478 could be considered an efficient, rapid, and easy method to make an early sex determination in the dioecious tree Ginkgo biloba.

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Morphological Characterisation And Molecular Sex Determination Of Human Remains From The 15th–17th Centuries In Latvia

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.2478/prolas-2014-0013 ◽

2015 ◽

Vol 69 (1-2) ◽

pp. 8-13 ◽

Cited By ~ 1

Author(s):

Asta Ščėsnaitė-Jerdiakova ◽

Liāna Pliss ◽

Guntis Gerhards ◽

Elīna Pētersone Gordina ◽

Agnija Gustiņa ◽

...

Keyword(s):

Sex Determination ◽

Dna Analysis ◽

Molecular Genetic ◽

Skeletal Remains ◽

Human Skeletal Remains ◽

Full Agreement ◽

Archaeological Material ◽

Molecular Approaches ◽

Molecular Sex Determination

Abstract Sex determination is one of the most important and initial steps in human profile identification from archaeological material. The aim of the current study was to evaluate the application of molecular approaches alongside morphological methods for sex determination in archaeological human skeletal remains. Human skeletal remains were excavated from three cemeteries: St Gertrude Old Church, Dom Square and St Peter’s Church, of 15th–17th century burials in Rīga, Latvia. Morphological and molecular genetic methods, including amplification of genes AMELX/Y and SRY were used to analyse seven skeletal remains. The conducted analyses of morphological features identified sex in all seven cases (two females and five males). By molecular analyses of mediaeval DNA it was possible to determine sex in five of seven (71%) samples. In all positive cases full agreement between morphological estimation and molecular genetic methods was observed. To conclude, DNA analysis can be considered for sex identification in cases with no signs of sexual dimorphism (juvenile skeletons) or partially preserved skeletons.

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Sex determination of human remains from peptides in tooth enamel

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1714926115 ◽

2017 ◽

Vol 114 (52) ◽

pp. 13649-13654 ◽

Cited By ~ 31

Author(s):

Nicolas Andre Stewart ◽

Raquel Fernanda Gerlach ◽

Rebecca L. Gowland ◽

Kurt J. Gron ◽

Janet Montgomery

Keyword(s):

Sex Determination ◽

Dna Analysis ◽

Sex Chromosome ◽

Tooth Enamel ◽

Human Remains ◽

Routine Activity ◽

Biological Sex ◽

Os Coxae ◽

First Time

The assignment of biological sex to archaeological human skeletons is a fundamental requirement for the reconstruction of the human past. It is conventionally and routinely performed on adults using metric analysis and morphological traits arising from postpubertal sexual dimorphism. A maximum accuracy of ∼95% is possible if both the cranium and os coxae are present and intact, but this is seldom achievable for all skeletons. Furthermore, for infants and juveniles, there are no reliable morphological methods for sex determination without resorting to DNA analysis, which requires good DNA survival and is time-consuming. Consequently, sex determination of juvenile remains is rarely undertaken, and a dependable and expedient method that can correctly assign biological sex to human remains of any age is highly desirable. Here we present a method for sex determination of human remains by means of a minimally destructive surface acid etching of tooth enamel and subsequent identification of sex chromosome-linked isoforms of amelogenin, an enamel-forming protein, by nanoflow liquid chromatography mass spectrometry. Tooth enamel is the hardest tissue in the human body and survives burial exceptionally well, even when the rest of the skeleton or DNA in the organic fraction has decayed. Our method can reliably determine the biological sex of humans of any age using a body tissue that is difficult to cross-contaminate and is most likely to survive. The application of this method will make sex determination of adults and, for the first time, juveniles a reliable and routine activity in future bioarcheological and medico-legal science contexts.

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Sex determination of skeletal remains of 4000 year old children and juveniles from Hoštice 1 za Hanou (Czech Republic) by ancient DNA analysis

Anthropological Review ◽

10.2478/v10044-008-0011-7 ◽

2008 ◽

Vol 71 (1) ◽

pp. 63-70 ◽

Cited By ~ 4

Author(s):

Michaela Vaňharová ◽

Eva Drozdová

Keyword(s):

Czech Republic ◽

Sex Determination ◽

Ancient Dna ◽

Dna Analysis ◽

Molecular Genetic ◽

Skeletal Remains ◽

Burial Site ◽

Ancient Dna Analysis ◽

Bell Beaker

Sex determination of skeletal remains of 4000 year old children and juveniles from Hoštice 1 za Hanou (Czech Republic) by ancient DNA analysisThe aim of this study was to determine the sex by means of modern molecular genetic methods of children and immature individuals from the 4000 years old Eneolithic burial site "Hoštice 1 za Hanou" of the Bell-Beaker people, in central Moravia (Czech Republic). While the anthropological approach was in this case limited either by the state of preservation of the skeletal remains or simply by absence of definite morphological traits in the children, analysis of aDNA (SRY, amelogenin) yielded results consistent with archeological grave findings and body imposition. The burial rites of the investigated culture facilitated the analysis because the gender specific imposition of adults has previously been described (man left-side, head northwards, woman right-side, head southwards) However, this approach is often limited in case of children burials. This study showed high concordance between archeological sex-determination and genetic sex, but also revealed several exceptions in children burial rite of Bell Beaker culture.

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