Study of the optimizing karyotype by applying AC voltage

Background: The main problem in the conventional karyotype slide is twisted and folded chromosomes that make it challenging to analyze the karyotype slide. Most of the time karyotype sorting software mistake chromosome with each order. In this study, the alternating current (AC) voltage conducted in order to drag the chromosome on karyotype slides. This method can facilitate the analysis of chromosomes structure by researchers, and karyotype sorting software can perform better and show a more accurate result. Methods: In this study, the AC ramp voltage has been applied to drag the chromosome on karyotype slides. AC voltage interacts with chromosomes in the slide so the force of electric charge can untwist and unfold chromosomes partially. Results: Karyotype chromosomes that were under AC voltage showed somewhat lined, and arranged chromosomes compare to conventional karyotype chromosomes. Conclusions: This method makes the study of the chromosomes structure very easy and more reliable. Hopefully, with the design and development of custom signal generators, the results of the study will be better and more accurate.