scholarly journals Pasteurella multocida Serotype 1 Isolated from a Lesser Snow Goose: Evidence of a Carrier State

1997 ◽  
Vol 33 (2) ◽  
pp. 332-335 ◽  
Author(s):  
M. D. Samuel ◽  
D. R. Goldberg ◽  
D. J. Shadduck ◽  
J. I. Price ◽  
E. G. Cooch
2017 ◽  
Vol 81 (5) ◽  
pp. 846-857 ◽  
Author(s):  
Jerry W. Hupp ◽  
David H. Ward ◽  
Kyle R. Hogrefe ◽  
James S. Sedinger ◽  
Philip D. Martin ◽  
...  

Nature ◽  
1987 ◽  
Vol 326 (6111) ◽  
pp. 392-394 ◽  
Author(s):  
Thomas W. Quinn ◽  
James S. Quinn ◽  
Fred Cooke ◽  
Bradley N. White

The Auk ◽  
1931 ◽  
Vol 48 (3) ◽  
pp. 335-364 ◽  
Author(s):  
George Miksch Sutton

The Auk ◽  
1916 ◽  
Vol 33 (2) ◽  
pp. 197-198
Author(s):  
Charles W. Townsend
Keyword(s):  

The Condor ◽  
2002 ◽  
Vol 104 (2) ◽  
pp. 432-436 ◽  
Author(s):  
Jason D. Weckstein ◽  
Alan D. Afton ◽  
Robert M. Zink ◽  
Ray T. Alisauskas

AbstractWe reanalyzed Quinn's (1992) mtDNA control region data set including new sequences from nine Lesser Snow Geese (Chen caerulescens caerulescens) and 10 Ross's Geese (Chen rossi) and found the same divergent lineages that Quinn (1992) attributed to vicariant separation of Lesser Snow Goose populations during the Pleistocene. However, peculiar patterns of mtDNA control region sequence variation, including a multimodal mismatch distribution of mtDNA sequences with two levels of population structuring and the sharing of two divergent haplotype lineages, are consistent with two hybridization episodes in Chen geese. Comparisons of mtDNA variation with historical and allozyme data sets compiled by Cooke et al. (1988) are consistent with the hypothesis that sharing of two mtDNA haplotype lineages between Ross's Goose and Lesser Snow Goose resulted from hybridization (Avise et al. 1992). Furthermore, population structure found within one haplotype cluster is consistent with Cooke et al.‘s (1988) hypothesis of past allopatry between blue and white Lesser Snow Geese.Hibridización y Subdivisión dentro y entre Poblaciones de Chen rossi y Chen caerulescens caerulescens: Una Perspectiva MolecularResumen. Reanalizamos los datos de la región de control del ADN mitocondrial (ADNmt) de Quinn (1992), junto con nuevas secuencias de nueve individuos de la especie Chen caerulescens caerulescens y 10 de Chen rossi. Encontramos los mismos linajes divergentes que Quinn (1992) atribuyó a la separación vicariante de las poblaciones de C. c. caerulescens durante el Pleistoceno. Sin embargo, encontramos que las dos especies comparten dos linajes de haplotipos divergentes, y la distribución de “mismatch” en secuencias del ADNmt mostró multimodalidad con dos niveles de estructuración de la población. Estos patrones peculiares están de acuerdo con la hipótesis de que hubo dos episodios de hibridización en gansos del género Chen. Los datos históricos y de aloenzimas compilados por Cooke et al. (1988) también apoyan esta hipótesis (Avise et al. 1992). Además, la estructura de la población dentro de un grupo de haplotipos es consistente con la hipótesis de Cooke et al. (1988) acerca de la pasada alopatría entre los morfos azul y blanco de C. c. caerulescens.


2005 ◽  
Vol 71 (11) ◽  
pp. 7187-7195 ◽  
Author(s):  
Robert E. Briggs ◽  
Fred M. Tatum

ABSTRACT Temperature-sensitive (TS) plasmids were generated through chemical mutagenesis of a derivative of the streptomycin resistance parent plasmid pD70, isolated from Mannheimia hemolytica serotype 1. Three TS plasmids which failed to replicate at or above 42°C in M. hemolytica but which were fully functional below 31°C were selected for further analysis. Two of the TS plasmids were shown by sequencing to possess unique single-base-pair mutations. The third TS plasmid contained a unique base pair substitution and a second mutation that had been previously identified. These mutations were clustered within a 200-bp region of the presumed plasmid origin of replication. Site-directed single-nucleotide substitutions were introduced into the wild-type pD70 origin of replication to confirm that mutations identified by sequencing had conferred thermoregulated replication. Deletion analysis on the wild-type pD70 plasmid replicon revealed that approximately 720 bp are necessary for plasmid maintenance. Replication of the TS plasmids was thermoregulated in Pasteurella multocida and Haemophilus somnus as well. To consistently transform H. somnus with TS plasmid, in vitro DNA methylation with commercially available HhaI methyltransferase was necessary to protect against the organism's restriction enzyme HsoI (recognition sequence 5′-GCGC-3′) characterized herein.


Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1723-1734 ◽  
Author(s):  
Ramakrishnan Srikumar ◽  
Leonie G. Mikael ◽  
Peter D. Pawelek ◽  
Ali Khamessan ◽  
Bernard F. Gibbs ◽  
...  

From the porcine pathogen Actinobacillus pleuropneumoniae cultivated in iron-deficient or haem-deficient media, haemoglobin (Hb)-agarose affinity purification was exploited to isolate an outer-membrane protein of ∼105 kDa, designated HgbA. Internal peptide sequences of purified HgbA were used to design oligonucleotide primers for PCR amplification, yielding amplicons that showed partial sequences with homology to hgbA of Pasteurella multocida. Upon screening two genomic libraries of A. pleuropneumoniae serotype 1 strain 4074, positive clones were assembled into an ORF of 2838 bp. HgbA (946 aa) includes a signal peptide of 23 aa and the deduced HgbA sequence (104 890 Da) also demonstrated a possible Ton box. The promoter region of hgbA from A. pleuropneumoniae serotype 1 showed consensus for −35 and −10 sequences and a putative Fur-binding site. RT-PCR confirmed that hgbA of A. pleuropneumoniae is upregulated in response to diminished levels of iron in the culture medium. While an internally deleted hgbA mutant was unable to use pig Hb as sole source of iron for growth, flow cytometry confirmed its Hb binding; the internally deleted sequences may not be required for Hb binding, but appear necessary for the iron supply from Hb. HgbA is required for growth of A. pleuropneumoniae in the presence of Hb as sole iron source.


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