Antimutagenic Activity of Cassia Auriculata Linn Fractions along with Anticancer Activity in Male Albino Mice

Background: In recent years, there has been a surge in interest in studying plant-derived materials and their impact on DNA. Herbal products include a number of natural substances that may help protect cells against mutagen-induced cell damage. Aim: The purpose of this research was to assess the genotoxic effects of Cassia Auriculata Linn flavonoids (CAF) and Cassia Auriculata Linn saponin (CAS) rich fractions on mouse bone marrow cells utilizing chromosomal aberration test and micronucleus assay. Methodology: The suppressive impact of CAF and CAS on 7, 12-dimethylbenz (α) anthracene (DMBA) and Croton oil induced skin tumor promotion in mice with topical administration twice weekly for 18 weeks is also investigated in this work. Three dosages of 100 and 200 mg/kg body weight were used. Single oral dosages of CAF and CAS Fraction at the three levels did not enhance the number of micronucleate polychromatic erythrocytes in the micronucleus experiment. Result: In mice bone marrow cells, a single oral treatment of CAF and CAS fraction revealed no significant alterations in mitotic indices or chromosomal aberration induction. The clastogenicity of CYP was considerably decreased by pretreatment with CAF and CAS fraction. As a result, it can be stated that CAF and CAS fraction had no genotoxic impact on mouse bone marrow cells. Conclusions: The portions of Cassia Auriculata have been shown to be non-genotoxic and non-clastogenic at the quantities utilized in this investigation. CAF and CAS Fraction might possibly be a promising skin tumor promotion reducing agent, according to this research.

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In Vivo Test for Chemical Induction of Micronucleated Polychromatic Erythrocytes in Mouse Bone Marrow Cells, Test Article: Ethylenediamine Dinitrate (EDDN)

10.21236/ada518233 ◽

2009 ◽

Author(s):

Karen Shore ◽

Paul Kirby

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Mouse Bone Marrow ◽

Chemical Induction ◽

In Vivo Test ◽

Test Article ◽

Polychromatic Erythrocytes ◽

Mouse Bone Marrow Cells ◽

Marrow Cells

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Effects of black pepper on the incidence of chromosomal aberration in mouse bone marrow cells in vivo.

CYTOLOGIA ◽

10.1508/cytologia.56.43 ◽

1991 ◽

Vol 56 (1) ◽

pp. 43-46 ◽

Cited By ~ 4

Author(s):

Annie T. John ◽

Susan Abraham

Keyword(s):

Bone Marrow ◽

Chromosomal Aberration ◽

Bone Marrow Cells ◽

Black Pepper ◽

Mouse Bone Marrow ◽

Mouse Bone Marrow Cells ◽

Marrow Cells

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Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

Toxicology and Industrial Health ◽

10.1177/0748233709345939 ◽

2009 ◽

Vol 25 (7) ◽

pp. 467-471 ◽

Cited By ~ 7

Author(s):

BN Mojidra ◽

K. Archana ◽

AK Gautam ◽

Y. Verma ◽

BC Lakkad ◽

...

Keyword(s):

Bone Marrow ◽

Chromosome Aberration ◽

Chromosomal Aberration ◽

Bone Marrow Cells ◽

Micronucleus Assay ◽

Control Group ◽

Genotoxic Potential ◽

Polychromatic Erythrocytes ◽

Significant Difference ◽

Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

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