[Multiparametric multivariate patch-clamp-spectroscopy as a method for plant signaling characterization and complementary systematic descriptor for biochemical taxonomy and biogeographic biochemical plant signaling mapping based on correlational phenospectal auxanometry]

Градов, О. В. Многофакторная патч-кламп-спектроскопия как метод характеризации сигнальных систем растений и источник комплементарных систематических дескрипторов для биохимической таксономии с привязкой к биогеографическим картам и феноспектральной ауксанометрии. In Сигнальные системы растений: от рецептора до ответной реакции организма (2016), Изд-во Санкт-Петербургского государственного университета СПб, pp. 79–81. =[Multiparametric multivariate patch-clamp-spectroscopy as a method for plant signaling characterization and complementary systematic descriptor for biochemical taxonomy and biogeographic biochemical plant signaling mapping based on correlational phenospectal auxanometry]

Biochemical taxonomy of Crenicichla (Pisces: Perciformes: Cichlidae) of the Iguaçu River, Brazil

Samples of Crenicichla iguassuensis and a morph from the Iguaçu River which differed from C. iguassuensis by its lips morphology were examined by isozyme starch electrophoresis technique. The analysis of 27 enzyme loci exhibited a genetic identity of 0.993 between the two morphs. No diagnostic locus was found that permitted to distinguish the two Crenicichla morphs. The data obtained in this investigation did not support the existence of two distinct species for this genus in the Iguaçu River, which suggested that the morphs stood for two polymorphic forms of Crenicichla iguassuensis.

Numerical taxonomy of Trypanozoon based on polymorphisms in a reduced range of enzymes

SUMMARYNumerical analyses of Trypanozoon taxonomy are presented, based on the isoenzyme data of Stevens et al. (1992). The previous study used a reduced range of enzymes compared with earlier work; the analyses indicate the value of this rationalized system. Both recently isolated trypanosome stocks and previously studied populations were included, allowing detailed comparison with earlier studies. Relationships between zymodemes were calculated with an improved similarity coefficient program, using Jaccard's coefficient (1908), and by Nei's method (1972). Dendrograms were constructed from the matrices produced with the group-average method. The groupings produced by both numerical methods were in close agreement, and the clusters of related principal zymodemes largely matched the species, subspecies and strain groups proposed by previous workers. Trypanozoon biochemical taxonomy is reviewed and the groupings reinforced by this study are: the mainly East African strain groups, busoga, zambezi, kakumbi, kiboko and sindo; T.b. gambiense and the bouaflé strain group from West Africa, and T. evansi; an intermediate bouaflé/busoga group was also recognized.

Nondestructive sampling of mitochondrial DNA from voles (Microtus)

We present two techniques for sampling mitochondrial DNA (mtDNA) without killing individual voles. Total cellular DNA was extracted from small blood samples (100–250 μL) and tail segments (2 cm long) collected from meadow voles (Microtus pennsylvanicus). Restriction fragment patterns produced by the restriction endonucleases HindIII, BamH1, and EcoR1 after hybridization with a probe of nick-translated mtDNA compared well with standard mtDNA assays. Both techniques can be used in the field, and should prove useful in biochemical taxonomy as well as in investigations of population structure, dispersal, and social interactions on both micro- and macro-geographic scales. The Southern blots produced by these procedures have the added advantage of being reuseable, so that nuclear DNA polymorphisms can be examined with appropriate probes.

The Subspecific Biochemical Taxonomy of Antechinus minimus, A. swainsonii and Sminthopsis leucopus (Marsupialia: Dasyuridae)

Electrophoretic variation in mainland and Tasmanian populations of Antechinus minimus, A. swainsonii and Sminthopsis leucopus was examined. The degree of genetic divergence observed among the trans-Bassian populations of each species corroborates the currently accepted subspecific rankings of A. minimus minimus and A. m. maritimus, A. swainsonii swainsonii and A. s. mimetes, and S. leucopus leucopus and S.l. ferruginifrons. The Flinders Island population of A. minimus was tentatively referred to the mainland subspecies, A. m. maritimus, on the basis of genetic similarity. Generally, levels of genetic divergence were found to be low when compared to those of similar taxonomic ranks in other animals.