Mobile Agent (MA) is the combine result of Agent technology and distributed technology. MA can move according to the hope of user; MA can take action independently according to the change of environment; MA can return the result after finishing the task. On the basis of analysis of the problems of traditional e-commerce, the design thought of E-Commerce based on MA is proposed. Combining the characteristics, object model, message mechanism and the common software package of IBM Aglet platform, the design framework of E-Commerce based on MA is put forward, the design aims, architecture, and concrete process, collaboration message between Multi-Agent, system safety program are confirmed. At last, the validity of the study is proved by the prototype system, and the key technologies in the implementation are discussed.