fluorescent particle
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2020 ◽  
Vol 48 (8) ◽  
pp. 948-950
Author(s):  
Holt S. Cutler ◽  
Jose A. Romero ◽  
David Minor ◽  
Michael H. Huo


BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 245-250
Author(s):  
Benjamin Lindner ◽  
Tobias Burkard ◽  
Michael Schuler

Phagocytosis is a fundamental mechanism of innate immunity and its impairment is associated with severe chronic diseases, for example, chronic obstructive pulmonary disease. Investigating phagocytosis requires flexible tools and assay conditions, such as different fluorescent particle types, detection colors and readouts. We comprehensively evaluated and optimized phagocytosis assays using particles labeled with fluorescent pH-sensitive pHrodo® dyes, facilitating the specific detection of phagocytosed particles. Beads, bacterial and yeast particles labeled with pHrodo red and green were tested for their uptake by THP-1 cells and primary human macrophages by flow cytometry and high-content imaging. Whereas the latter allowed kinetic phagocytosis measurement, the former demonstrated the feasibility of using cell sorting for periods of up to 6 h, enabling downstream applications such as pooled genetic screens.



2019 ◽  
Vol 34 (9) ◽  
pp. 2075-2079
Author(s):  
David C. Holst ◽  
Marc R. Angerame ◽  
Douglas A. Dennis ◽  
Jason M. Jennings


Author(s):  
Y Y Zhang ◽  
Z W Cheng ◽  
Y C Jin ◽  
K Si ◽  
X Y Jin


Nanoscale ◽  
2019 ◽  
Vol 11 (29) ◽  
pp. 14050-14059 ◽  
Author(s):  
Hajar Masoomi ◽  
Yao Wang ◽  
Xiaoxia Fang ◽  
Peirui Wang ◽  
Cang Chen ◽  
...  

A novel ultrabright fluorescent particle based on dye-loaded polyelectrolyte brushes is successfully established and the structure-fluorescence tuning principles are revealed.



2016 ◽  
Vol 28 (12) ◽  
pp. 2421-2426 ◽  
Author(s):  
Paloma Rodríguez-Sevilla ◽  
Yuhai Zhang ◽  
Patricia Haro-González ◽  
Francisco Sanz-Rodríguez ◽  
Francisco Jaque ◽  
...  


2016 ◽  
Vol 4 (19) ◽  
pp. 3358-3364 ◽  
Author(s):  
Bin Liu ◽  
Xiangquan Hu ◽  
Jie Chai ◽  
Junyao Zhu ◽  
Binsheng Yang ◽  
...  

A nanodiamond-conjugated rhodamine fluorescent sensor for Cu(ii) which could then be developed as an excellent NO selective fluorescent particle.



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