hrp horseradish peroxidase
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scholarly journals Optimization of Aflatoxin B1 Aptasensing

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Marzieh Jafari ◽  
Mohsen Rezaei ◽  
Heibatullah Kalantari ◽  
Maryam Tabarzad ◽  
Bahram Daraei
Keyword(s):  
Horseradish Peroxidase ◽  
Binding Affinity ◽  
Aflatoxin B1 ◽  
Low Cost ◽  
Term Stability ◽  
Long Term Stability ◽  
Conventional Methods ◽  
User Friendly ◽  
Hrp Horseradish Peroxidase

Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps.

Linkage of boronated polylysine to glycoside moieties of polyclonal antibody; boronated antibodies as potential delivery agents for neutron capture therapy 1 1Abbreviations: BNCT, Boron neutron capture therapy; BPL, Boronated poly-DL-lysine; BSA, Bovine serum albumin; DMBA, p-Dimethylaminobenzaldehyde; DMF, Dimethylformamide; DMSO, Dimethyl sulfoxide; DTT, Dithiothreitol; FTSC, Fluorescein thiosemicarbazide; HRP, Horseradish peroxidase; IgG, Immunoglobulin-G; IRMA, Immunoradiometric assay; LET, Linear energy transfer; MB-hydrazide, m-Maleimidobenzoyl hydrazide; MW, Molecular weight; PBS, Phosphate buffered saline; PL, Poly-DL-lysine; SDS-PAGE, Sodium n-Dodecyl Sulfate Polyacrylamide Gel Electrophoresis; SPDP, N-Succinimidyl-3-(2 pyridyldithio) propionate; Sulfo-MBS, m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester; TRIZMA®, Tris (hydroxymethyl) aminomethane; TSH, Thyroid stimulating hormone.

2002 ◽  
Vol 29 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Sara Novick ◽  
Michael R Quastel ◽  
Stephanie Marcus ◽  
David Chipman ◽  
Gad Shani ◽  
...  
Keyword(s):  
Neutron Capture ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Thyroid Stimulating Hormone ◽  
Neutron Capture Therapy ◽  
Boron Neutron Capture ◽  
Sds Page ◽  
Dtt Dithiothreitol ◽  
Phosphate Buffered Saline ◽  
Hrp Horseradish Peroxidase
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