Pollutants Removal and Analysis of Microbial Community of Malodorous River in BFG-IVCWs

The changes of concentrations of pH, DO, CODCr, NH4+-N and NO3--N in Biofilm Grid-Integrated Vertical-flow Constructed Wetlands（BFG-IVCWs）by treating the urban malodorous river were measured. After 45 days, DO had its own distribution and changing regulation, the removal rate of CODCrreach to 45%, at meantime, the removal rate of NO3--N are over 50%. The diversity of microbial community and its succession were analyzed by PCR-DGGE fingerprint. The result shows: the removal rate of CODCrand NH4+-N along the river flow gradually rises, and the removal rate of NO3--N rises at last. The removal of pollutants mainly happened in the down-flow system of the BFG-IVCWs. The differences of characters of biofilm and environmental condition of different positions made the microbial community structure diversity variable. Three kinds of biofilms (W3, W5 and W7) were analyzed with Shannon-Wiener and Cs indices and the value in position W3 was the highest.

Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis

ABSTRACT Denaturing gradient gel electrophoresis (DGGE) is widely used in microbial ecology. We tested the effect of fluorophore-labeled primers on DGGE band migration, sensitivity, and normalization. The fluorophores Cy5 and Cy3 did not visibly alter DGGE fingerprints; however, 6-carboxyfluorescein retarded band migration. Fluorophore modification improved the sensitivity of DGGE fingerprint detection and facilitated normalization of samples from multiple gels by the application of intralane standards.