In this chapter, a unified web accessibility assessment (UWAA) framework and its software has been proposed. UWAA framework was developed by considering Web Content Accessibility Guideline 2.0 to evaluate accessibility of web sites by integrating more than one evaluation approach. Achecker tool as an automated evaluation approach and barrier walkthrough (BW) as an expert-based evaluation approach were integrated in the UWAA framework. The framework also provides suggestions to recover from the problems determined to the evaluators. The websites of three universities were evaluated to determine the framework's accuracy and consistency. It was revealed that the results obtained from automated and expert-based evaluation methods were consistent and complementary with each other. Furthermore, it has been demonstrated that problems which cannot be determined by an automated tool but which can be detected by an expert can be identified by BW method.