The prevention and the therapy of such diseases as atherosclerosis, hepatitis, and cancer is nowadays one of the most significant medical problems. Dodecyl(3,5-dimethyl-4-hydroxybenzyl)sulfide was synthesized at the chair for chemistry of Novosibirsk State Pedagogical University. This substance is a new promising phenolic antioxidant, which can be used in the complex therapy of abovementioned diseases. The aim of this study was to develop methods for quantification of dodecyl(3,5-dimethyl-4-hydroxybenzyl)sulfide for standardization and bioanalytical studies. The mercurimetric titration, HPLC-UV and voltammetry were used. The methods validation was performed according to general pharmacopoeial monograph OFS.1.1.0012.15 "Analytical methods validation" and OFS.1.1.0013.15 "Statistical processing of chemical experiment results" of the Russian State Pharmacopoeia XIV. All the results meet the acceptance criteria in parameters specificity, linearity, precision and accuracy. The mercurimetric titration of dodecyl(3,5-dimethyl-4-hydroxybenzyl)sulfide is recommended for including in chapter "Assay" of the original monograph project for substance. The HPLC-UV method may be useful for the determination of the object of study in dosage forms. The voltammetric method is promising for bioanalytical researches.
Development of Methods for Quantitative Determination of The New Antioxidant Dodecyl(3,5-Dimethyl-4-Hydroxybenzyl)Sulfide