fiasco protocol
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2014 ◽  
Vol 13 (1) ◽  
pp. 100-104 ◽  
Author(s):  
Meng WANG ◽  
Fei XUE ◽  
Peng YANG ◽  
Xia-yu DUAN ◽  
Yi-lin ZHOU ◽  
...  

HortScience ◽  
2010 ◽  
Vol 45 (2) ◽  
pp. 314-315 ◽  
Author(s):  
Hai-fei Yan ◽  
Xue-jun Ge ◽  
Chi-ming Hu ◽  
Gang Hao

Nine microsatellite loci were isolated from Primula obconica using the FIASCO protocol. We used 30 individuals from three populations for the assessment of microsatellite variation. Seven loci were detected with microsatellite polymorphism. The number of alleles per locus ranged from three to seven. The average observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.6 and from 0.409 to 0.653, respectively. These microsatellite markers will be useful to assess the genetic variation and genetic structure of P. obconica.


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