Determination of Membrane Protein Molecular Weight Using Sedimentation Equilibrium Analytical Ultracentrifugation

2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Karen G. Fleming
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Analytical Ultracentrifugation ◽  
Sedimentation Equilibrium ◽  
Protein Molecular Weight

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

1990 ◽  
Vol 10 (2) ◽  
pp. 131-139
Author(s):  
Oyewole Adeyemo ◽  
E. O. Okegbile ◽  
O. O. Olorunsogo
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Partial Purification ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
Triton X

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

Quantitative determination of protein molecular weight with an acoustic sensor; significance of specific versus non-specific binding

The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 3918-3925 ◽  
Author(s):  
Konstantinos Mitsakakis ◽  
Achilleas Tsortos ◽  
Electra Gizeli
Keyword(s):  
Molecular Weight ◽  
Quantitative Determination ◽  
Phase Change ◽  
Acoustic Signal ◽  
Specific Binding ◽  
Acoustic Sensor ◽  
Protein Molecular Weight

A multi-analyte acoustic biosensor determines the molecular weight of proteinsviathe phase change of the acoustic signal.

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